Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GADD34 antibody [OTI2B11] (ab236516)

Key features and details

  • Mouse monoclonal [OTI2B11] to GADD34
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Dog, Human, Recombinant fragment
  • Isotype: IgG1

Overview

  • Product name

    Anti-GADD34 antibody [OTI2B11]
    See all GADD34 primary antibodies

  • Description

    Mouse monoclonal [OTI2B11] to GADD34

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Recombinant fragment
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human GADD34 aa 1 to the C-terminus. Produced in HEK-293T cells (NP_055145).
    Database link: O75807

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: pCMV6-ENTRY GADD34 cDNA transfected HEK-293T cell lysate; HepG2, HeLa, SVT2, A549 and MCF7 cell lysates. ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY GADD34. Flow Cyt: HEK-293T cells transfected with GADD34; HeLa and Jurkat cells.

  • General notes

    Clone OTI2B11 (formerly 2B11).

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-GADD34 antibody [OTI2B11] (ab236516)
    Western blot - Anti-GADD34 antibody [OTI2B11] (ab236516)
    All lanes : Anti-GADD34 antibody [OTI2B11] (ab236516) at 1/500 dilution

    Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : pCMV6-ENTRY GADD34 cDNA transfected HEK-293T cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 73 kDa

  • Western blot - Anti-GADD34 antibody [OTI2B11] (ab236516)
    Western blot - Anti-GADD34 antibody [OTI2B11] (ab236516)
    All lanes : Anti-GADD34 antibody [OTI2B11] (ab236516) at 1/500 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 3 : SVT2 cell lysate
    Lane 4 : A549 (human lung carcinoma cell line) cell lysate
    Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell lysate
    Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 7 : MDCK (canine kidney cell line) cell lysate
    Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
    Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 73 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-GADD34 antibody [OTI2B11] (ab236516)
    Immunocytochemistry/ Immunofluorescence - Anti-GADD34 antibody [OTI2B11] (ab236516)

    COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY GADD34 stained for GADD34 (green) using ab236516 at 1/100 dilution in ICC/IF.

  • Flow Cytometry - Anti-GADD34 antibody [OTI2B11] (ab236516)
    Flow Cytometry - Anti-GADD34 antibody [OTI2B11] (ab236516)

    Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell line transfected with either GADD34 overexpress plasmid (Red) or empty vector control plasmid (Blue) labeling GADD34 with ab236516 at 1/100 dilution.

  • Flow Cytometry - Anti-GADD34 antibody [OTI2B11] (ab236516)
    Flow Cytometry - Anti-GADD34 antibody [OTI2B11] (ab236516)

    Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling GADD34 with ab236516 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).

  • Flow Cytometry - Anti-GADD34 antibody [OTI2B11] (ab236516)
    Flow Cytometry - Anti-GADD34 antibody [OTI2B11] (ab236516)

    Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling GADD34 with ab236516 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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