Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Phosphotyrosine antibody [Py72] - BSA and Azide free (ab269516)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [Py72] to Phosphotyrosine - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Phosphotyrosine antibody [Py72] - BSA and Azide free
    See all Phosphotyrosine primary antibodies

  • Description

    Mouse monoclonal [Py72] to Phosphotyrosine - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Other Immunogen Type. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Pervanadate treated NIH/3T3, Daudi and MCF7 whole cell lysates. IP: Pervanadate treated NIH/3T3 and MCF7 whole cell lysates.

  • General notes

    ab269516 is the carrier-free version of ab269515. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab269516 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Phosphotyrosine antibody [Py72] - BSA and Azide free (ab269516)
    Western blot - Anti-Phosphotyrosine antibody [Py72] - BSA and Azide free (ab269516)
    All lanes : Human Omentin ELISA Kit (ITLN1) (ab269545) at 0.196 µg/ml

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 2 : NIH/3T3 treated with 10mM pervanadate for 20 minutes, whole cell lysates
    Lane 3 : Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate
    Lane 4 : Daudi treated with 10mM pervanadate for 30 minutes, whole cell lysates
    Lane 5 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 6 : MCF7 treated with 1mM pervanadate for 20 minutes, whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/100000 dilution


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269515).

    Multiple bands represent phosph-tyrosine containing proteins detected by ab269515.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-4: 3.25 secs; Lanes 5-6: 3 mins.

  • Immunoprecipitation - Anti-Phosphotyrosine antibody [Py72] - BSA and Azide free (ab269516)
    Immunoprecipitation - Anti-Phosphotyrosine antibody [Py72] - BSA and Azide free (ab269516)

    Phosphotyrosine was immunoprecipitated from MCF7 (human breast adenocarcinoma epithelial cell), treated with 1mM pervanadate for 20 minutes, whole cell lysates, with ab269515 at 1/30 dilution (2µg in 0.35mg lysates, (32.667µg/ml)). Western blot was performed on the immunoprecipitate using ab269515 at 1/1000 dilution (0.196µg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: MCF7 treated with 1mM pervanadate for 20 mins, whole cell lysates, 10µg.

    Lane 2: ab269515 IP in MCF7 treated with 1mM pervanadate for 20 mins, whole cell lysates.

    Lane 3: Mouse monoclonal IgG1 (ab18443) instead of ab269515 in MCF7 treated with 1mM pervanadate for 20 mins, whole cell lysates.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    Multiple bands represent phosph-tyrosine containing proteins precipitated and detected by ab269515.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269515).

  • Immunoprecipitation - Anti-Phosphotyrosine antibody [Py72] - BSA and Azide free (ab269516)
    Immunoprecipitation - Anti-Phosphotyrosine antibody [Py72] - BSA and Azide free (ab269516)

    Phosphotyrosine was immunoprecipitated from NIH/3T3 (Mouse embyro fibroblast cells), treated with 10mM pervanadate for 20 minutes, whole cell lysates, with ab269515 at 1/30 dilution (2µg in 0.35mg lysates, (32.667µg/ml)). Western blot was performed on the immunoprecipitate using ab269515 at 1/1000 dilution (0.196µg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 treated with 10mM pervanadate for 20 mins, whole cell lysates, 10µg.

    Lane 2: ab269515 IP in NIH/3T3 treated with 10mM pervanadate for 20 mins, whole cell lysates.

    Lane 3: Mouse monoclonal IgG1 (ab18443) instead of ab269515 in NIH/3T3 treated with 10mM pervanadate for 20 mins, whole cell lysates.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    Multiple bands represent phosph-tyrosine containing proteins precipitated and detected by ab269515.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269515).

  • Anti-Phosphotyrosine antibody [Py72] - BSA and Azide free (ab269516)
    Anti-Phosphotyrosine antibody [Py72] - BSA and Azide free (ab269516)

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