Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TMPRSS2 antibody [EPR3861] - BSA and Azide free (ab239905)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3861] to TMPRSS2 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TMPRSS2 antibody [EPR3861] - BSA and Azide free
    See all TMPRSS2 primary antibodies

  • Description

    Rabbit monoclonal [EPR3861] to TMPRSS2 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Caco 2, LNCaP and PC3 whole cell lysates. Human heart, brain, colon, prostate, and small intestine tissue lysates. Mouse prostate and kidney tissue lysates. Rat colon and kidney tissue lysates; IHC-P: Human prostatic adenocarcinoma and human kidney.

  • General notes

    Ab239905 is the carrier-free version of ab92323. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239905 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-TMPRSS2 antibody [EPR3861] - BSA and Azide free (ab239905)
    Western blot - Anti-TMPRSS2 antibody [EPR3861] - BSA and Azide free (ab239905)
    All lanes : Anti-TMPRSS2 antibody [EPR3861] (ab92323) at 1/1000 dilution

    Lane 1 : LnCaP cell lysate
    Lane 2 : Human prostate tissue lysate
    Lane 3 : Caco 2 cell lysate
    Lane 4 : Human lung tissue lysate
    Lane 5 : Human heart tissue lysate
    Lane 6 : Human brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 54 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab92323).

    Lanes 1 - 6: Merged signal (red and green). Green - ab92323 observed at 52 kDa. Red - loading control, mouse anti GAPDH observed at 37kDa.

    ab92323 was shown to react with TMPRSS2 in western blot. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab92323 and mouse anti GAPDH overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS2 antibody [EPR3861] - BSA and Azide free (ab239905)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS2 antibody [EPR3861] - BSA and Azide free (ab239905)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue sections labeling TMPRSS2 with purified ab92323 at 1/1000 dilution (0.49 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92323).
  • Western blot - Anti-TMPRSS2 antibody [EPR3861] - BSA and Azide free (ab239905)
    Western blot - Anti-TMPRSS2 antibody [EPR3861] - BSA and Azide free (ab239905)
    All lanes : Anti-TMPRSS2 antibody [EPR3861] (ab92323) at 1/1000 dilution (purified)

    Lane 1 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate
    Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : Human colon lysate
    Lane 4 : Human small intestine lysate
    Lane 5 : Mouse prostate lysate
    Lane 6 : Mouse kidney lysate
    Lane 7 : Rat colon lysate
    Lane 8 : Rat kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92323).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS2 antibody [EPR3861] - BSA and Azide free (ab239905)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS2 antibody [EPR3861] - BSA and Azide free (ab239905)

    Unpurified ab92323 at 1/1000 dilution, staining TMPRSS2 in paraffin embedded Human prostatic adenocarcinoma

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92323).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-TMPRSS2 antibody [EPR3861] - BSA and Azide free (ab239905)
    Anti-TMPRSS2 antibody [EPR3861] - BSA and Azide free (ab239905)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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