Anti-Vitamin D Binding protein antibody [EP2923Y] - BSA and Azide free (ab247521)
![Anti-Vitamin D Binding protein antibody [EP2923Y] - BSA and Azide free (ab247521)](https://www.abcam.com/ps/products/247/ab247521/Images/ab247521-405650-anti-vitamin-d-binding-protein-antibody-ep2923y-bsa-and-azide-free-western-blot.jpg "Anti-Vitamin D Binding protein antibody [EP2923Y] - BSA and Azide free (ab247521)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2923Y] to Vitamin D Binding protein - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-Vitamin D Binding protein antibody [EP2923Y] - BSA and Azide free
See all Vitamin D Binding protein primary antibodies -
Description
Rabbit monoclonal [EP2923Y] to Vitamin D Binding protein - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247521 is the carrier-free version of ab81307.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2923Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Vitamin D Binding protein antibody [EP2923Y] - BSA and Azide free (ab247521)Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307) at 1/20000 dilution + Human plasma at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab81307, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Binding protein antibody [EP2923Y] - BSA and Azide free (ab247521)](https://www.abcam.com/ps/products/247/ab247521/Images/ab247521-1-anti-vitamin-d-binding-protein-antibody-ep2923y-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Binding protein antibody [EP2923Y] - BSA and Azide free (ab247521)This data was developed using ab81307, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human liver using ab81307 at a 1/100 dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. -
![Anti-Vitamin D Binding protein antibody [EP2923Y] - BSA and Azide free (ab247521)](https://www.abcam.com/ps/products/247/ab247521/Images/ab247521-3-benefits-of-recombinant-antibodies.png)
Anti-Vitamin D Binding protein antibody [EP2923Y] - BSA and Azide free (ab247521)