Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FXR1 antibody [EPR7932] - BSA and Azide free (ab240042)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7932] to FXR1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-FXR1 antibody [EPR7932] - BSA and Azide free
    See all FXR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7932] to FXR1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Hep G2, A549, Hela, HEK-293

  • General notes

    ab240042 is the carrier-free version of ab129089.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-FXR1 antibody [EPR7932] - BSA and Azide free (ab240042)
    Immunocytochemistry/ Immunofluorescence - Anti-FXR1 antibody [EPR7932] - BSA and Azide free (ab240042)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling FXR1 with purified ab129089 at 1/500 dilution. Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG (Alexa Fluor®488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129089).

  • Flow Cytometry - Anti-FXR1 antibody [EPR7932] - BSA and Azide free (ab240042)
    Flow Cytometry - Anti-FXR1 antibody [EPR7932] - BSA and Azide free (ab240042)

    ab129089 staining FXR1 in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/60. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129089).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FXR1 antibody [EPR7932] - BSA and Azide free (ab240042)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FXR1 antibody [EPR7932] - BSA and Azide free (ab240042)

    ab129089, at a dilution of 1/100, staining FXR1 in paraffin-embedded Human brain tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129089).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-FXR1 antibody [EPR7932] - BSA and Azide free (ab240042)
    Immunocytochemistry/ Immunofluorescence - Anti-FXR1 antibody [EPR7932] - BSA and Azide free (ab240042)
    ab129089, at a dilution of 1/250, staining Hela cells by immunofluorescent staining

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129089).

  • OI-RD Scanning - Anti-FXR1 antibody [EPR7932] - BSA and Azide free (ab240042)
    OI-RD Scanning - Anti-FXR1 antibody [EPR7932] - BSA and Azide free (ab240042)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129089).

  • Anti-FXR1 antibody [EPR7932] - BSA and Azide free (ab240042)
    Anti-FXR1 antibody [EPR7932] - BSA and Azide free (ab240042)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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