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Anti-FXR1 antibody [EPR7932] (ab129089)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7932] to FXR1
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-FXR1 antibody [EPR7932]
    See all FXR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7932] to FXR1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues near the N-terminus of Human FXR1 (UniProt ID: P51114).

  • Positive control

    • Fetal muscle, MCF7, 293T, HeLa, and fetal heart lysates, Human brain tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-FXR1 antibody [EPR7932] (ab129089)
    Western blot - Anti-FXR1 antibody [EPR7932] (ab129089)
    All lanes : Anti-FXR1 antibody [EPR7932] (ab129089) at 1/1000 dilution

    Lane 1 : Fetal muscle lysate
    Lane 2 : MCF7 lysate
    Lane 3 : 293T lysate
    Lane 4 : HeLa lysate
    Lane 5 : Fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 70 kDa
    Observed band size: 70-78 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-FXR1 antibody [EPR7932] (ab129089)
    Immunocytochemistry/ Immunofluorescence - Anti-FXR1 antibody [EPR7932] (ab129089)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling FXR1 with purified ab129089 at 1/500 dilution. Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG (Alexa Fluor®488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Flow Cytometry - Anti-FXR1 antibody [EPR7932] (ab129089)
    Flow Cytometry - Anti-FXR1 antibody [EPR7932] (ab129089)

    ab129089 staining FXR1 in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/60. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FXR1 antibody [EPR7932] (ab129089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FXR1 antibody [EPR7932] (ab129089)

    ab129089, at a dilution of 1/100, staining FXR1 in paraffin-embedded Human brain tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-FXR1 antibody [EPR7932] (ab129089)
    Immunocytochemistry/ Immunofluorescence - Anti-FXR1 antibody [EPR7932] (ab129089)
    ab129089, at a dilution of 1/250, staining Hela cells by immunofluorescent staining
  • OI-RD Scanning - Anti-FXR1 antibody [EPR7932] (ab129089)
    OI-RD Scanning - Anti-FXR1 antibody [EPR7932] (ab129089)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-FXR1 antibody [EPR7932] (ab129089)
    Anti-FXR1 antibody [EPR7932] (ab129089)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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