Anti-CHD4 antibody [EPR12229] - BSA and Azide free (ab250495)
![Anti-CHD4 antibody [EPR12229] - BSA and Azide free (ab250495)](https://www.abcam.com/ps/products/250/ab250495/Images/ab250495-405892-anti-chd4-antibody-epr12229-bsa-and-azide-free-western-blot.jpg "Anti-CHD4 antibody [EPR12229] - BSA and Azide free (ab250495)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12229] to CHD4 - BSA and Azide free
- Suitable for: ICC, WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CHD4 antibody [EPR12229] - BSA and Azide free
See all CHD4 primary antibodies -
Description
Rabbit monoclonal [EPR12229] to CHD4 - BSA and Azide free -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 95% homology with CDH3. Cross-reactivity with this protein has not been confirmed experimentally.
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Tested applications
Suitable for: ICC, WB, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250495 is the carrier-free version of ab181370 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab250495 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR12229 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CHD4 antibody [EPR12229] - BSA and Azide free (ab250495)All lanes : Anti-CHD4 antibody [EPR12229] (ab181370) at 1/10000 dilution
Lane 1 : NCCIT cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 218 kDaThis data was developed using ab181370, the same antibody clone in a different buffer formulation.
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![Immunocytochemistry - Anti-CHD4 antibody [EPR12229] - BSA and Azide free (ab250495)](https://www.abcam.com/ps/products/250/ab250495/Images/ab250495-2-anti-chd4-antibody-epr12229-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-CHD4 antibody [EPR12229] - BSA and Azide free (ab250495) -
![Flow Cytometry - Anti-CHD4 antibody [EPR12229] - BSA and Azide free (ab250495)](https://www.abcam.com/ps/products/250/ab250495/Images/ab250495-1-anti-chd4-antibody-epr12229-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-CHD4 antibody [EPR12229] - BSA and Azide free (ab250495) -
![Anti-CHD4 antibody [EPR12229] - BSA and Azide free (ab250495)](https://www.abcam.com/ps/products/250/ab250495/Images/ab250495-4-benefits-of-recombinant-antibodies.png)
Anti-CHD4 antibody [EPR12229] - BSA and Azide free (ab250495)
