Anti-CHD4 antibody [3F2/4] - ChIP Grade – BSA and Azide free (ab264521)
![Anti-CHD4 antibody [3F2/4] - ChIP Grade – BSA and Azide free (ab264521)](https://www.abcam.com/ps/products/264/ab264521/Images/ab264521-356298-ab70469260297antichd4antibody3f24chipgradeimmunohistochemistry.jpg "Anti-CHD4 antibody [3F2/4] - ChIP Grade – BSA and Azide free (ab264521)")
Key features and details
- Mouse monoclonal [3F2/4] to CHD4 - ChIP Grade – BSA and Azide free
- Suitable for: IP, ELISA, IHC-P, Flow Cyt, ChIP, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-CHD4 antibody [3F2/4] - ChIP Grade – BSA and Azide free
See all CHD4 primary antibodies -
Description
Mouse monoclonal [3F2/4] to CHD4 - ChIP Grade – BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IP, ELISA, IHC-P, Flow Cyt, ChIP, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
-
Immunogen
Synthetic peptide corresponding to Human CHD4.
-
Positive control
- IHC: Human breast carcinoma tissue.
-
General notes
ab264521 is the carrier-free version of ab70469.If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
3F2/4 -
Isotype
IgG1 -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD4 antibody [3F2/4] - ChIP Grade – BSA and Azide free (ab264521)
IHC image of CHD4 staining in human breast carcinoma formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70469, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab70469).
