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Immunology Immune System Diseases Autoimmune

Anti-CHD3 antibody [EPNCIR110] (ab134125)

Price and availability

311 587 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-CHD3 antibody [EPNCIR110] (ab134125)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPNCIR110] to CHD3
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-CHD3 antibody [EPNCIR110]
    See all CHD3 primary antibodies
  • Description

    Rabbit monoclonal [EPNCIR110] to CHD3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IHC-P
    Mouse
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Mouse CHD3.

  • Positive control

    • Mouse brain lysate, Raw264.7 cell lysate, NIH 3T3 cell lysate and cells, Mouse spleen tissue.
  • General notes

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Gordon Hager. View antibodies from NCI Center for Cancer Research Collaboration.

    Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPNCIR110
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immune System Diseases
    • Autoimmune
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Mi-2
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors

Images

  • Western blot - Anti-CHD3 antibody [EPNCIR110] (ab134125)
    Western blot - Anti-CHD3 antibody [EPNCIR110] (ab134125)
    All lanes : Anti-CHD3 antibody [EPNCIR110] (ab134125) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Raw264.7 cell lysate
    Lane 3 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 234 kDa
    Observed band size: 260 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110] (ab134125)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110] (ab134125)

    Immunohistochemical analysis of paraffin embedded Mouse spleen tissue labelling CHD3 with ab134125 at 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110] (ab134125)
    Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110] (ab134125)
    Immunofluorescent staining of 3T3 cells labelling CHD3 with ab134125 at 1/100.
  • Anti-CHD3 antibody [EPNCIR110] (ab134125)
    Anti-CHD3 antibody [EPNCIR110] (ab134125)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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