Anti-CHD3 antibody [EPNCIR110] (ab134125)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR110] to CHD3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse
Overview
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Product name
Anti-CHD3 antibody [EPNCIR110]
See all CHD3 primary antibodies -
Description
Rabbit monoclonal [EPNCIR110] to CHD3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseIHC-P MouseWB Mouse -
Immunogen
Synthetic peptide corresponding to Mouse CHD3.
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Positive control
- Mouse brain lysate, Raw264.7 cell lysate, NIH 3T3 cell lysate and cells, Mouse spleen tissue.
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General notes
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Gordon Hager. View antibodies from NCI Center for Cancer Research Collaboration.
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPNCIR110 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-CHD3 antibody [EPNCIR110] (ab134125) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 234 kDa
Observed band size: 260 kDa why is the actual band size different from the predicted?
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Immunohistochemical analysis of paraffin embedded Mouse spleen tissue labelling CHD3 with ab134125 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent staining of 3T3 cells labelling CHD3 with ab134125 at 1/100.
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