Anti-Hsp27 (phospho S78) antibody (ab131467)
Key features and details
- Rabbit polyclonal to Hsp27 (phospho S78)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Hsp27 (phospho S78) antibody
See all Hsp27 primary antibodies -
Description
Rabbit polyclonal to Hsp27 (phospho S78) -
Host species
Rabbit -
Specificity
ab131467 detects Hsp27 only when phosphorylated at serine 78. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Hsp27 conjugated to keyhole limpet haemocyanin.
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Positive control
- HeLa cells; extracts from HL60 cells; Human breast carcinoma tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab131467 was purified by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Hsp27 (phospho S78) antibody (ab131467) at 1/500 dilution
Lane 1 : Extract from HL60 cells, untreated
Lane 2 : Extract from HL60 cells treated with UV
Predicted band size: 23 kDa
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Immunocytochemistry/ Immunofluorescence analysis of Hsp27 (phospho S78) in methanol fixed HeLa cells labelled with ab131467 at a 1/100 dilution.
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Immunohistochemical analysis of Hsp27 (phospho S78) in paraffin embedded Human breast carcinoma cells labelled with ab131467 at a 1/50 dilution. The image on the right was preincubated with blocking peptide.