Anti-Hsp27 (phospho S15) antibody [EP2293Y] - BSA and Azide free (ab247464)
![Anti-Hsp27 (phospho S15) antibody [EP2293Y] - BSA and Azide free (ab247464)](https://www.abcam.com/ps/products/247/ab247464/Images/ab247464-407921-anti-hsp27-phospho-s15-antibody-ep2293y-bsa-and-azide-free-western-blot.jpg "Anti-Hsp27 (phospho S15) antibody [EP2293Y] - BSA and Azide free (ab247464)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2293Y] to Hsp27 (phospho S15) - BSA and Azide free
- Suitable for: ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-Hsp27 (phospho S15) antibody [EP2293Y] - BSA and Azide free
See all Hsp27 primary antibodies -
Description
Rabbit monoclonal [EP2293Y] to Hsp27 (phospho S15) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody will detect Hsp27 phosphorylation on Serine 15.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247464 is the carrier-free version of ab76313 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247464 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2293Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Hsp27 (phospho S15) antibody [EP2293Y] - BSA and Azide free (ab247464)All lanes : Anti-Hsp27 (phospho S15) antibody [EP2293Y] (ab76313) at 1/2500 dilution
Lane 1 : Untreated HeLa (human cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 23 kDa
Additional bands at: 27 kDa. We are unsure as to the identity of these extra bands.This data was developed using ab76313, the same antibody clone in a different buffer formulation.
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![Immunocytochemistry - Anti-Hsp27 (phospho S15) antibody [EP2293Y] - BSA and Azide free (ab247464)](https://www.abcam.com/ps/products/247/ab247464/Images/ab247464-1-anti-hsp27-phospho-s15-antibody-ep2293y-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-Hsp27 (phospho S15) antibody [EP2293Y] - BSA and Azide free (ab247464)This data was developed using ab76313, the same antibody clone in a different buffer formulation.ab76313 staining Hsp27 (phospho S15) in HeLa (human cervix adenocarcinoma) cells with heat treatment at 44°C. by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/150. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab76313 (1/1000) and secondary antibody ab150077 (1/1000) respectively and DAPI was used as a nuclear counterstain. Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) -
![Anti-Hsp27 (phospho S15) antibody [EP2293Y] - BSA and Azide free (ab247464)](https://www.abcam.com/ps/products/247/ab247464/Images/ab247464-3-benefits-of-recombinant-antibodies.png)
Anti-Hsp27 (phospho S15) antibody [EP2293Y] - BSA and Azide free (ab247464)
