Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hsp27 (phospho S15) antibody [EP2293Y] - BSA and Azide free (ab247464)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2293Y] to Hsp27 (phospho S15) - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Hsp27 (phospho S15) antibody [EP2293Y] - BSA and Azide free
    See all Hsp27 primary antibodies

  • Description

    Rabbit monoclonal [EP2293Y] to Hsp27 (phospho S15) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody will detect Hsp27 phosphorylation on Serine 15. 

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247464 is the carrier-free version of ab76313 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247464 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Hsp27 (phospho S15) antibody [EP2293Y] - BSA and Azide free (ab247464)
    Western blot - Anti-Hsp27 (phospho S15) antibody [EP2293Y] - BSA and Azide free (ab247464)
    All lanes : Anti-Hsp27 (phospho S15) antibody [EP2293Y] (ab76313) at 1/2500 dilution

    Lane 1 : Untreated HeLa (human cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 23 kDa
    Additional bands at: 27 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab76313, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-Hsp27 (phospho S15) antibody [EP2293Y] - BSA and Azide free (ab247464)
    Immunocytochemistry - Anti-Hsp27 (phospho S15) antibody [EP2293Y] - BSA and Azide free (ab247464)
    This data was developed using ab76313, the same antibody clone in a different buffer formulation.ab76313 staining Hsp27 (phospho S15) in HeLa (human cervix adenocarcinoma) cells with heat treatment at 44°C. by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/150. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab76313 (1/1000) and secondary antibody ab150077 (1/1000) respectively and DAPI was used as a nuclear counterstain. Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)
  • Anti-Hsp27 (phospho S15) antibody [EP2293Y] - BSA and Azide free (ab247464)
    Anti-Hsp27 (phospho S15) antibody [EP2293Y] - BSA and Azide free (ab247464)

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