Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EGF antibody [EPR19173] - BSA and Azide free (ab240283)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19173] to EGF - BSA and Azide free
  • Suitable for: WB, IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-EGF antibody [EPR19173] - BSA and Azide free
    See all EGF primary antibodies

  • Description

    Rabbit monoclonal [EPR19173] to EGF - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Fr, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab240283 is the carrier-free version of ab184265.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR19173] - BSA and Azide free (ab240283)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR19173] - BSA and Azide free (ab240283)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling EGF with ab184265 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on distal convoluted tubules of mouse kidney [PMID 1930151]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184265).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR19173] - BSA and Azide free (ab240283)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR19173] - BSA and Azide free (ab240283)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling EGF with ab184265 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on distal convoluted tubules of rat kidney [PMID 1930151]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184265).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR19173] - BSA and Azide free (ab240283)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR19173] - BSA and Azide free (ab240283)

    Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling EGF with ab184265 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    No staining on mouse stomach.

    Counter stained with Hematoxylin.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184265).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR19173] - BSA and Azide free (ab240283)
    Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR19173] - BSA and Azide free (ab240283)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling EGF with ab184265 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Positive staining on mouse kidney was observed. 

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184265).

  • Anti-EGF antibody [EPR19173] - BSA and Azide free (ab240283)
    Anti-EGF antibody [EPR19173] - BSA and Azide free (ab240283)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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