Anti-EGF antibody [EPR19174] - Low endotoxin, Azide free (ab223549)
![Anti-EGF antibody [EPR19174] - Low endotoxin, Azide free (ab223549)](https://www.abcam.com/ps/products/223/ab223549/Images/ab223549-321422-anti-egf-antibody-epr19174-immunohistochemistry.jpg "Anti-EGF antibody [EPR19174] - Low endotoxin, Azide free (ab223549)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19174] to EGF - Low endotoxin, Azide free
- Suitable for: IP, IHC-Fr, WB
- Reacts with: Mouse
Overview
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Product name
Anti-EGF antibody [EPR19174] - Low endotoxin, Azide free
See all EGF primary antibodies -
Description
Rabbit monoclonal [EPR19174] to EGF - Low endotoxin, Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse EGF recombinant protein; Mouse salivary gland and kidney lysates; Mouse urine sample. IHC-Fr: Mouse kidney tissue. IP: Mouse urine sample
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General notes
ab223549 is the carrier-free version of ab184266.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19174 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR19174] - Low endotoxin, Azide free (ab223549)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver tissue labeling EGF with ab184266 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green).
Weak staining on mouse liver is observed. The expression profile observed is consistent with what has been described in the literature (PMID:17671655).
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184266).
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![Immunoprecipitation - Anti-EGF antibody [EPR19174] - Low endotoxin, Azide free (ab223549)](https://www.abcam.com/ps/products/223/ab223549/Images/ab223549-321421-anti-egf-antibody-epr19174-immunoprecipitation.jpg)
Immunoprecipitation - Anti-EGF antibody [EPR19174] - Low endotoxin, Azide free (ab223549)EGF was immunoprecipitated from 0.35 mg of Mouse urine sample with ab184266 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184266 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse urine sample, 10µg (Input).
Lane 2: ab184266 IP in Mouse urine sample.
Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab184266 in Mouse urine sample.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184266).
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![Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR19174] - Low endotoxin, Azide free (ab223549)](https://www.abcam.com/ps/products/223/ab223549/Images/ab223549-282801-anti-egf-antibody-epr19174-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR19174] - Low endotoxin, Azide free (ab223549)This IHC data was generated using the same anti-EGF antibody clone [EPR19174] in a different buffer formulation (cat# ab184266).
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling EGF with ab184266 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Cytoplasmic staining on mouse kidney is observed. The staining pattern is consistent with what has been described in the literature (PMID:17671655).
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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![Anti-EGF antibody [EPR19174] - Low endotoxin, Azide free (ab223549)](https://www.abcam.com/ps/products/223/ab223549/Images/ab223549-4-benefits-of-recombinant-antibodies.png)
Anti-EGF antibody [EPR19174] - Low endotoxin, Azide free (ab223549)
