Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7411-6] to eIF5A2 + eIF5A - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free
    See all eIF5A2 + eIF5A primary antibodies

  • Description

    Rabbit monoclonal [EPR7411-6] to eIF5A2 + eIF5A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248154 is the carrier-free version of ab126735. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248154 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
    Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
    All lanes : Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Caco-2 cell lysate
    Lane 5 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 17 kDa



    This data was developed using ab126735, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
    Flow Cytometry - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)

    This data was developed using ab126735, the same antibody clone in a different buffer formulation.

    Overlay histogram showing HeLa cells stained with ab126735 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126735, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)

    This data was developed using ab126735, the same antibody clone in a different buffer formulation.

    ab126735 at 1/100 dilution, staining eIF5A2 + eIF5A in Formalin fixed Paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
    Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
    All lanes : Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735) at 1/2000 dilution

    Lane 1 : Recombinant Human eIF5A protein (ab87457)
    Lane 2 : Recombinant Human eIF5A2 protein (ab99140)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 17 kDa
    Observed band size: 15,20 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 seconds


    Blocking buffer: 5% NFDM/TBST

    This data was developed using ab126735, the same antibody clone in a different buffer formulation.
  • OI-RD Scanning - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
    OI-RD Scanning - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
    This data was developed using ab126735, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
    Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)

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