Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] (ab224696)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22492-2] to KIR2DL1 + KIR2DL2
  • Suitable for: IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2]

  • Description

    Rabbit monoclonal [EPR22492-2] to KIR2DL1 + KIR2DL2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged human KIR2DL1 recombinant protein (aa1-245); His and LIF-tagged human KIR2DL2 recombinant protein (aa26-221). IHC-P: Human endometrium tissue. ICC/IF: NK-92 cells (treated with 5-aza-2-deoxycytidine (2µM) for 48h). Flow Cyt: Human PBMCs. IP: NK-92 (treated with 2µM 5-aza-2-deoxycytidine for 48h) whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22492-2

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] (ab224696)
    Immunocytochemistry/ Immunofluorescence - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] (ab224696)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) cells (+/- treatment with 5-aza-2-deoxycytidine(2µM) for 48h) labeling KIR2DL1 + KIR2DL2 with ab224696 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in NK-92 cells treated with 5-aza-2-deoxycytidine(2uM) for 48h. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] (ab224696)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] (ab224696)

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling KIR2DL1 + KIR2DL2 with ab224696 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in natural killer (NK) cells in human endometrium (PMID: 7749980) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunoprecipitation - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] (ab224696)
    Immunoprecipitation - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] (ab224696)

    KIR2DL1 + KIR2DL2 were immunoprecipitated from 0.35 mg of NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) (treated with 2μM 5-aza-2-deoxycytidine for 48h) whole cell lysate with ab224696 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224696 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: NK-92 (treated as above) whole cell lysate 10 μg (Input).
    Lane 2: ab224696 IP in NK-92 (treated as above) whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab224696 in NK-92 (treated as above) whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 100 seconds.

    This blot was developed using a higher sensitivity ECL substrate.

  • Flow Cytometry - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] (ab224696)
    Flow Cytometry - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] (ab224696)

    Flow cytometric analysis of human PBMCs (peripheral blood mononuclear cells) labeling KIR2DL1 + KIR2DL2 with ab224696 at 1/60 dilution (Right) compared with Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left).

    Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or ab224696 (Right). Then stained with anti-CD56 conjugated to BV421.

    Gated on viable cells.

     

  • Western blot - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] (ab224696)
    Western blot - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] (ab224696)
    All lanes : Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] (ab224696) at 1/1000 dilution

    Lane 1 : His-tagged human KIR2DL1 recombinant protein (aa1-245) 20 ng
    Lane 2 : His and LIF-tagged human KIR2DL2 recombinant protein (aa26-221) 20 ng
    Lane 3 : His-tagged human KIR2DL3 recombinant protein (aa1-245) 20 ng
    Lane 4 : His-tagged human KIR2DS1 recombinant protein (aa22-221) 20 ng
    Lane 5 : His-tagged human KIR2DS2 recombinant protein (aa22-221) 20 ng
    Lane 6 : His-tagged human KIR2DS3 recombinant protein (aa22-221) 20 ng
    Lane 7 : His-tagged human KIR2DS4 recombinant protein (aa22-221) 20 ng
    Lane 8 : His-tagged human KIR2DS5 recombinant protein (aa22-221) 20 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 39 kDa


    Exposure time: 26 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] (ab224696)
    Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] (ab224696)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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