Antibodies, Proteins, Kits and Reagents for Life Science

Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7412-50] to eIF5A2 + eIF5A - BSA and Azide free
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free
See all eIF5A2 + eIF5A primary antibodies
Description

Rabbit monoclonal [EPR7412-50] to eIF5A2 + eIF5A - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Recombinant Human eIF5A2 protein (ab99140) can be used as a positive control in WB. HepG2, HeLa, 293T and Raji cell lysates; Human colon adenocarcinoma and testis tissues.
General notes
ab271915 is the carrier-free version of ab150439. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm.

Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR7412-50
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)

Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labelling eIF5A2 + eIF5A with ab150439 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation (ab150439).
Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)

All lanes : Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439) at 1/1000 dilution

Lane 1 : Recombinant Human eIF5A protein (ab87457)
Lane 2 : Recombinant Human eIF5A2 protein (ab99140)

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 17 kDa
Observed band size: 15,20 kDa
why is the actual band size different from the predicted?

Exposure time: 7 seconds

Blocking buffer: 5% NFDM/TBST

This data was developed using ab150439, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling eIF5A2 + eIF5A with ab150439 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation (ab150439).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab150439 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation (ab150439).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)

Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150439 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation (ab150439).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)

Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab150439 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation (ab150439).
Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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