Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Thursday March 04, 2021

Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7412-50] to eIF5A2 + eIF5A
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-eIF5A2 + eIF5A antibody [EPR7412-50]
See all eIF5A2 + eIF5A primary antibodies
Description

Rabbit monoclonal [EPR7412-50] to eIF5A2 + eIF5A
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human eIF5A2 + eIF5A aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary. The immunogen sequence is based upon a region of eIF5A2 that has high homology with eIF5A.
Database link: Q9GZV4
Positive control
- Recombinant Human eIF5A2 protein (ab99140) can be used as a positive control in WB. HepG2, HeLa, 293T and Raji cell lysates; Human colon adenocarcinoma and testis tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR7412-50
Isotype

IgG
Research areas

Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439)

All lanes : Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439) at 1/1000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Raji cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP labelled antibody at 1/2000 dilution

Predicted band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439)

Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labelling eIF5A2 + eIF5A with ab150439 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439)

All lanes : Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439) at 1/1000 dilution

Lane 1 : Recombinant Human eIF5A protein (ab87457)
Lane 2 : Recombinant Human eIF5A2 protein (ab99140)

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 17 kDa
Observed band size: 15,20 kDa
why is the actual band size different from the predicted?

Exposure time: 7 seconds

Blocking buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling eIF5A2 + eIF5A with ab150439 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439)

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab150439 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2+eIF5A antibody [EPR7412-50] (ab150439)

Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150439 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439)

Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab150439 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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