Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7412-47] to eIF5A2 + eIF5A
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-eIF5A2 + eIF5A antibody [EPR7412-47]
    See all eIF5A2 + eIF5A primary antibodies

  • Description

    Rabbit monoclonal [EPR7412-47] to eIF5A2 + eIF5A

  • Host species

    Rabbit

  • Specificity

    The antibody may react with not only A2 but also A1.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human eIF5A2 + eIF5A aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary. The immunogen sequence is based upon a region of eIF5A2 that has high homology with eIF5A.
    Database link: Q9GZV4

  • Positive control

    • HepG2 and Caco 2 cell lysates; Human ovarian carcinoma tissue; HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
    Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
    All lanes : Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Caco 2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)

    ab126733 at 1/100 dilution, staining eIF5A2 + eIF5A in Formalin-fixed Paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)

    ab126733, at 1/100 dilution, staining eIF5A2 + eIF5A in HepG2 cells by Immunofluorescence (green). DAPI counterstain (blue).

  • Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
    Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
    All lanes : Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733) at 1/1000 dilution

    Lane 1 : Recombinant Human eIF5A protein (ab87457)
    Lane 2 : Recombinant Human eIF5A2 protein (ab99140)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 17 kDa
    Observed band size: 15,20 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking buffer: 5% NFDM/TBST
  • OI-RD Scanning - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
    OI-RD Scanning - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
    Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)

© 2021 Astana Biomed Group LLP. All rights reserved.