Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ERGIC3 antibody [EPR8141] - BSA and Azide free (ab248334)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8141] to ERGIC3 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ERGIC3 antibody [EPR8141] - BSA and Azide free
    See all ERGIC3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8141] to ERGIC3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, HepG2, MCF7, K562 and HeLa cell lysates. IHC-P: Human brain tissue. ICC: HeLa cells.

  • General notes

    Ab248334 is the carrier-free version of ab129179. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248334 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ERGIC3 antibody [EPR8141] - BSA and Azide free (ab248334)
    Western blot - Anti-ERGIC3 antibody [EPR8141] - BSA and Azide free (ab248334)
    All lanes : Anti-ERGIC3 antibody [EPR8141] (ab129179) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : ERGIC3 knockout HEK293T cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : MCF-7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 43 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab129179, the same antibody clone in a different buffer formulation.

    Lanes 1-4: Merged signal (red and green). Green - ab129179 observed at 50 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab129179 Anti-ERGIC3 antibody [EPR8141] was shown to specifically react with ERGIC3 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266489 (knockout cell lysate ab257941) was used. Wild-type and ERGIC3 knockout samples were subjected to SDS-PAGE. ab129179 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC3 antibody [EPR8141] - BSA and Azide free (ab248334)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC3 antibody [EPR8141] - BSA and Azide free (ab248334)
    This data was developed using ab129179, the same antibody clone in a different buffer formulation.ab129179, at 1/100, staining ERGIC3 in paraffin embedded Human brain tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Western blot - Anti-ERGIC3 antibody [EPR8141] - BSA and Azide free (ab248334)
    Western blot - Anti-ERGIC3 antibody [EPR8141] - BSA and Azide free (ab248334)
    All lanes : Anti-ERGIC3 antibody [EPR8141] (ab129179) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Predicted band size: 43 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    This data was developed using ab129179, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-ERGIC3 antibody [EPR8141] - BSA and Azide free (ab248334)
    Immunocytochemistry - Anti-ERGIC3 antibody [EPR8141] - BSA and Azide free (ab248334)
    This data was developed using ab129179, the same antibody clone in a different buffer formulation.ab129179, at 1/250, staining ERGIC3 in HeLa cells by Immunofluorescence.
  • OI-RD Scanning - Anti-ERGIC3 antibody [EPR8141] - BSA and Azide free (ab248334)
    OI-RD Scanning - Anti-ERGIC3 antibody [EPR8141] - BSA and Azide free (ab248334)
    This data was developed using ab129179, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-ERGIC3 antibody [EPR8141] - BSA and Azide free (ab248334)
    Anti-ERGIC3 antibody [EPR8141] - BSA and Azide free (ab248334)

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