Anti-Nucleophosmin antibody (ab31319)
Key features and details
- Goat polyclonal to Nucleophosmin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Nucleophosmin antibody
See all Nucleophosmin primary antibodies -
Description
Goat polyclonal to Nucleophosmin -
Host species
Goat -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Dog, Chimpanzee
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Immunogen
Synthetic peptide:
C-QEAIQDLWQWRKSL
, corresponding to C terminal amino acids 281-294 of Human Nucleophosmin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Nucleophosmin antibody (ab31319)Lane 1 : Anti-Nucleophosmin antibody (ab31319) at 1 µg/ml
Lane 2 : Anti-Nucleophosmin antibody (ab31319) at 0.1 µg/ml
Lanes 3-4 : Anti-Nucleophosmin antibody (ab31319) at 0.01 µg/ml
Lane 1 : NIH3T3
Lane 2 : Daudi
Lane 3 : Jurkat
Lane 4 : K562
Lysates/proteins at 35 µg per lane.Detected by chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody (ab31319)Immunocytochemistry/ Immunofluorescence of HeLa cell labeling nucleophosmin with ab31319 at 10 µg/mL.Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. Alexa Fluor488 at 2 µg/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor488 secondary antibody (2 µg/mL). -
Western blot - Anti-Nucleophosmin antibody (ab31319)Anti-Nucleophosmin antibody (ab31319) at 1 µg/ml + Mouse spleen tissue lysate at 35 µgDetected by chemiluminescence.
