Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Nucleophosmin antibody [EP1848Y] (ab52644)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1848Y] to Nucleophosmin
Suitable for: ICC/IF, WB, Flow Cyt, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Nucleophosmin antibody [EP1848Y]
See all Nucleophosmin primary antibodies
Description

Rabbit monoclonal [EP1848Y] to Nucleophosmin
Host species

Rabbit
Specificity

The mouse and rat recommendation is based on the Western Blot results. We do not guarantee Immunohistochemistry for mouse and rat.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Nucleophosmin aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P06748
Positive control
- WB: HeLa, MEF, PC-12, 3T3-L1 cell lysates; IHC-P: Human cervical carcinoma tissue; ICC/IF: HeLa cells; Flow Cyt: HeLa cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1848Y
Isotype

IgG
Research areas

Western blot - Anti-Nucleophosmin antibody [EP1848Y] (ab52644)

Anti-Nucleophosmin antibody [EP1848Y] (ab52644) at 1/20000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 33 kDa
Observed band size: 250,27,33 kDa
why is the actual band size different from the predicted?

The band at 250kDa might be an oligomer and the band at 33kDa might be an isoform of NPM.

Blocking/Diluting buffer: 5% NFDM/TBST
Flow Cytometry - Anti-Nucleophosmin antibody [EP1848Y] (ab52644)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells, labeling Nucleophosmin with Purified ab52644 at 1:40 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-Nucleophosmin antibody [EP1848Y] (ab52644)

All lanes : Anti-Nucleophosmin antibody [EP1848Y] (ab52644) at 1/500000 dilution

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : MEF (Mouse embryonic fibroblast) whole cell lysate
Lane 3 : 3T3-L1 (Mouse embryonic fibroblast) whole cell lysate
Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 33 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [EP1848Y] (ab52644)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue sections labeling Nucleophosmin with purified ab52644 at 1/300 dilution (1.14 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [EP1848Y] (ab52644)

Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Nucleophosmin with purified ab52644 at 1/100 dilution (5 µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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