Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

50 µl 335 040 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Nucleophosmin antibody [FC82291] (ab10530)

Key features and details

Mouse monoclonal [FC82291] to Nucleophosmin
Suitable for: ICC/IF, WB
Reacts with: Mouse, Rat, Cow, Dog, Human, African green monkey, Chinese hamster
Isotype: IgG1

Product name

Anti-Nucleophosmin antibody [FC82291]
See all Nucleophosmin primary antibodies
Description

Mouse monoclonal [FC82291] to Nucleophosmin
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
WB	Mouse Rat Cow Dog Human African green monkey Chinese hamster

See all applications and species data

Immunogen

Full length native protein (purified) (Rat).
Epitope

The epitope recognized by the antibody lies within the 68 amino acids at the C terminus of B23.
Positive control
- NIH3T3 whole cell lysate.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA
Concentration information loading...
Purity

Protein G purified
Clonality

Monoclonal
Clone number

FC82291
Isotype

IgG1
Research areas

Western blot - Anti-Nucleophosmin antibody [FC82291] (ab10530) This image is courtesy of an anonymous Abreview

Western blot image of ab10530 staining whole cell lysate of U2OS cells. The gel was blocked with 5% milk for 1 hour at 21°C. The primary antibody was diluted to 2 µg/ml and incubated for 12 hours at 4°C. A HRP conjugated goat anti-mouse antibody was used as the secondary.
See Abreview
Western blot - Anti-Nucleophosmin antibody [FC82291] (ab10530)

Anti-Nucleophosmin antibody [FC82291] (ab10530) at 0.2 µg/ml + 3T3 cell lysate

Secondary
Anti-Mouse IgG (Fab)

Predicted band size: 33 kDa
Western blot - Anti-Nucleophosmin antibody [FC82291] (ab10530)

All lanes : Anti-Nucleophosmin antibody [FC82291] (ab10530) at 0.5 µg/ml

Lane 1 : HeLa cell lysate
Lane 2 : JURKAT cell lysate
Lane 3 : COS7 cell lysate
Lane 4 : NIH-3T3 cell lysate
Lane 5 : RAT2 cell lysate
Lane 6 : CHO cell lysate
Lane 7 : MDBK cell lysate
Lane 8 : MDCK cell lysate

Secondary
All lanes : Goat Anti-Mouse IgG-Peroxidase

Predicted band size: 33 kDa

Cell line lysate separated on SDS-PAGE, probed with ab10530 (0.5 μg/mL)
Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [FC82291] (ab10530)

Immunocytochemistry/ Immunofluorescence analysis of HeLa cells staining Nucleophosmin using ab10530 (dilution 1/500). Cells were fixed and permeabilized with methanol followed by methanol:acetone. Developed using Goat Anti-Mouse IgG, Cy3™ conjugate and counterstained with DAPI (blue) to stain nuclei.
Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [FC82291] (ab10530) This image is courtesy of an Anonymous Abreview.

ab10530 staining Nucleophosmin in immortalized Human trabecular meshwork cells by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 3% BSA for 45 minutes at room temperature. The primary antibody was diluted 1/1000 in PBS/0.3% BSA and incubated with the sample for 2 hours. The secondary antibody was Cy3^®-conjugated Donkey anti-Mouse polyclonal, diluted 1/1000.
See Abreview

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