Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (ab109546)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1856] to Nucleophosmin (phospho S125)
Suitable for: Flow Cyt, WB, IHC-P
Reacts with: Human

Product name

Anti-Nucleophosmin (phospho S125) antibody [EPR1856]
See all Nucleophosmin primary antibodies
Description

Rabbit monoclonal [EPR1856] to Nucleophosmin (phospho S125)
Host species

Rabbit
Specificity

Detects Nucleophosmin only if phosphorylated at Serine 125.

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Phospho-peptide corresponding to residues surrounding Serine 125 of Human Nucleophosmin
Positive control
- WB: HeLa cell lysate IHC-P: Human breast or kidney tissue
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR1856
Isotype

IgG
Research areas

Western blot - Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (ab109546)

All lanes : Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (ab109546) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with Alkaline Phosphatase

Lysates/proteins at 10 µg per lane.

Predicted band size: 33 kDa
Flow Cytometry - Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (ab109546)

Overlay histogram showing HeLa cells stained with ab109546 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109546, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (ab109546)

ab109546, at a 1/100 dilution, staining Nucleophosmin in formailn-fixed, paraffin-embedded Human breast tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (ab109546)

ab109546, at a 1/100 dilution, staining Nucleophosmin in formalin-fixed, paraffin-embedded Human kidney tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (ab109546)

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