Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [CL0296] to RBM3
Suitable for: ICC/IF, IHC-P, WB
Reacts with: Human
Isotype: IgG1

Product name

Anti-RBM3 antibody [CL0296]
See all RBM3 primary antibodies
Description

Mouse monoclonal [CL0296] to RBM3
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human RBM3 aa 1 to the C-terminus.
Database link: P98179

Run BLAST with Run BLAST with
Positive control
- RT-4 cell lysate; Human breast cancer, colorectal tumor and prostate tumor tissues. WB: U-241 cells
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

CL0296
Isotype

IgG1
Research areas

Western blot - Anti-RBM3 antibody [CL0296] (ab211356)

All lanes : Anti-RBM3 antibody [CL0296] (ab211356)

Lane 1 : U-251 cells, transfected with control siRNA
Lane 2 : U-251 cells, transfected with target specific siRNA probe #1
Lane 3 : U-251 cells, transfected with target specific siRNA probe #2

Predicted band size: 17 kDa

Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM3 antibody [CL0296] (ab211356)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling RBM3 with ab211356 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-RBM3 antibody [CL0296] (ab211356)

Immunofluorescence staining of U-2 OS cells staining RBM3 useing ab211356, showing specific staining in the nucleoplasm in green. Microtubule are visualized in red. Antibody staining is shown in green.
Western blot - Anti-RBM3 antibody [CL0296] (ab211356)

Anti-RBM3 antibody [CL0296] (ab211356) at 1/500 dilution + RT-4 cell lysate

Predicted band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM3 antibody [CL0296] (ab211356)

Immunohistochemical analysis of paraffin-embedded Human colorectal tumor tissue labeling RBM3 with ab211356 at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM3 antibody [CL0296] (ab211356)

Immunohistochemical analysis of paraffin-embedded Human prostate tumor tissue labeling RBM3 with ab211356 at 1/500 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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