Anti-RBM3 antibody [CL0296] (ab211356)
![Anti-RBM3 antibody [CL0296] (ab211356)](https://www.abcam.com/ps/products/211/ab211356/Images/ab211356-281863-anti-rbm3-antibody-cl0296-western-blot.jpg "Anti-RBM3 antibody [CL0296] (ab211356)")
Key features and details
- Mouse monoclonal [CL0296] to RBM3
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-RBM3 antibody [CL0296]
See all RBM3 primary antibodies -
Description
Mouse monoclonal [CL0296] to RBM3 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human RBM3 aa 1 to the C-terminus.
Database link: P98179 -
Positive control
- RT-4 cell lysate; Human breast cancer, colorectal tumor and prostate tumor tissues. WB: U-241 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL0296 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-RBM3 antibody [CL0296] (ab211356)All lanes : Anti-RBM3 antibody [CL0296] (ab211356)
Lane 1 : U-251 cells, transfected with control siRNA
Lane 2 : U-251 cells, transfected with target specific siRNA probe #1
Lane 3 : U-251 cells, transfected with target specific siRNA probe #2
Predicted band size: 17 kDaDownregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM3 antibody [CL0296] (ab211356)](https://www.abcam.com/ps/products/211/ab211356/Images/ab211356-260022-anti-rbm3-antibody-cl0296-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM3 antibody [CL0296] (ab211356)Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling RBM3 with ab211356 at 1/500 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-RBM3 antibody [CL0296] (ab211356)](https://www.abcam.com/ps/products/211/ab211356/Images/ab211356-279289-anti-rbm3-antibody-cl0296-western-blot.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RBM3 antibody [CL0296] (ab211356)Immunofluorescence staining of U-2 OS cells staining RBM3 useing ab211356, showing specific staining in the nucleoplasm in green. Microtubule are visualized in red. Antibody staining is shown in green.
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![Western blot - Anti-RBM3 antibody [CL0296] (ab211356)](https://www.abcam.com/ps/products/211/ab211356/Images/ab211356-260019-anti-rbm3-antibody-cl0296-western-blot.jpg)
Western blot - Anti-RBM3 antibody [CL0296] (ab211356)Anti-RBM3 antibody [CL0296] (ab211356) at 1/500 dilution + RT-4 cell lysate
Predicted band size: 17 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM3 antibody [CL0296] (ab211356)](https://www.abcam.com/ps/products/211/ab211356/Images/ab211356-260021-anti-rbm3-antibody-cl0296-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM3 antibody [CL0296] (ab211356)Immunohistochemical analysis of paraffin-embedded Human colorectal tumor tissue labeling RBM3 with ab211356 at 1/500 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM3 antibody [CL0296] (ab211356)](https://www.abcam.com/ps/products/211/ab211356/Images/ab211356-260020-anti-rbm3-antibody-cl0296-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM3 antibody [CL0296] (ab211356)Immunohistochemical analysis of paraffin-embedded Human prostate tumor tissue labeling RBM3 with ab211356 at 1/500 dilution.
