Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RBM3 antibody [EPR6061(2)] - BSA and Azide free (ab240089)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6061(2)] to RBM3 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RBM3 antibody [EPR6061(2)] - BSA and Azide free
    See all RBM3 primary antibodies

  • Description

    Rabbit monoclonal [EPR6061(2)] to RBM3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WB, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab188553)

  • Positive control

    • ICC: NIH/3T3 cells; IHC-P: Rat and mouse liver, and human astrocytoma tissues; Flow Cyt (intra): HepG2 cells; WB: Jurkat, HeLa, Neuro-2a, HEK-293T, LnCap, NIH/3T3 and PC-12 whole cell lysates.

  • General notes

    ab240089 is the carrier-free version of ab134946.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-RBM3 antibody [EPR6061(2)] - BSA and Azide free (ab240089)
    Western blot - Anti-RBM3 antibody [EPR6061(2)] - BSA and Azide free (ab240089)
    All lanes : Anti-RBM3 antibody [EPR6061(2)] (ab134946) at 1/1000 dilution (Purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 17 kDa



    We are unsure how to define the extra bands.

  • Immunocytochemistry - Anti-RBM3 antibody [EPR6061(2)] - BSA and Azide free (ab240089)
    Immunocytochemistry - Anti-RBM3 antibody [EPR6061(2)] - BSA and Azide free (ab240089)
    This data was developed using ab134946, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling RBM3 with Purified ab134946 at 1/100 dilution (1.32 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM3 antibody [EPR6061(2)] - BSA and Azide free (ab240089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM3 antibody [EPR6061(2)] - BSA and Azide free (ab240089)
    This data was developed using ab134946, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling RBM3 with Purified ab134946 at 1/300 dilution (0.44 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow Cytometry - Anti-RBM3 antibody [EPR6061(2)] - BSA and Azide free (ab240089)
    Flow Cytometry - Anti-RBM3 antibody [EPR6061(2)] - BSA and Azide free (ab240089)
    This data was developed using ab134946, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling RBM3 with Purified ab134946 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM3 antibody [EPR6061(2)] - BSA and Azide free (ab240089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM3 antibody [EPR6061(2)] - BSA and Azide free (ab240089)
    This data was developed using ab134946, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling RBM3 with Purified ab134946 at 1/300 dilution (0.44 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM3 antibody [EPR6061(2)] - BSA and Azide free (ab240089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM3 antibody [EPR6061(2)] - BSA and Azide free (ab240089)
    This data was developed using ab134946, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human astrocytoma tissue sections labeling RBM3 with Purified ab134946 at 1/300 dilution (0.44 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • OI-RD Scanning - Anti-RBM3 antibody [EPR6061(2)] - BSA and Azide free (ab240089)
    OI-RD Scanning - Anti-RBM3 antibody [EPR6061(2)] - BSA and Azide free (ab240089)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134946).

  • Anti-RBM3 antibody [EPR6061(2)] - BSA and Azide free (ab240089)
    Anti-RBM3 antibody [EPR6061(2)] - BSA and Azide free (ab240089)

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