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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases

Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] - BSA and Azide free (ab187408)

Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] - BSA and Azide free (ab187408)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [34.2] to AMPK alpha 1 + AMPK alpha 2 - BSA and Azide free
  • Suitable for: WB, IP, ELISA, IHC-Fr, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] - BSA and Azide free
    See all AMPK alpha 1 + AMPK alpha 2 primary antibodies
  • Description

    Mouse monoclonal [34.2] to AMPK alpha 1 + AMPK alpha 2 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IP, ELISA, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Drosophila melanogaster AMPK alpha 1 + AMPK alpha 2 (N terminal).

  • Positive control

    • WB: Rat heart lysate; HepG2 and HEK-293T whole cell lysates.
  • General notes

    ab187408 is a PBS only version of ab80039.

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    34.2
  • Isotype

    IgG2a
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Neuroscience
    • Neurology process
    • Metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Images

  • Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] - BSA and Azide free (ab187408)
    Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] - BSA and Azide free (ab187408)
    All lanes : Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab80039) at 1 µg/ml

    Lane 1 : Heart (Rat) Tissue Lysate
    Lane 2 : ab29466
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 62 kDa
    Observed band size: 62 kDa
    Additional bands at: 75 kDa, 80 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    This data was developed using the same antibody clone in a different formulation containing PBS, Azide and arginine (ab80039).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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