Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] - BSA and Azide free (ab187408)
Key features and details
- Mouse monoclonal [34.2] to AMPK alpha 1 + AMPK alpha 2 - BSA and Azide free
- Suitable for: WB, IP, ELISA, IHC-Fr, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] - BSA and Azide free
See all AMPK alpha 1 + AMPK alpha 2 primary antibodies -
Description
Mouse monoclonal [34.2] to AMPK alpha 1 + AMPK alpha 2 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, IP, ELISA, IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Drosophila melanogaster AMPK alpha 1 + AMPK alpha 2 (N terminal).
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Positive control
- WB: Rat heart lysate; HepG2 and HEK-293T whole cell lysates.
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General notes
ab187408 is a PBS only version of ab80039.
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
34.2 -
Isotype
IgG2a -
Research areas
Images
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All lanes : Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab80039) at 1 µg/ml
Lane 1 : Heart (Rat) Tissue Lysate
Lane 2 : ab29466
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 62 kDa
Observed band size: 62 kDa
Additional bands at: 75 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesThis data was developed using the same antibody clone in a different formulation containing PBS, Azide and arginine (ab80039).