HRP Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab202685)
![HRP Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab202685)](https://www.abcam.com/ps/products/202/ab202685/Images/ab202685-249531-IHCAP24094411.jpg "HRP Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab202685)")
Key features and details
- HRP Mouse monoclonal [34.2] to AMPK alpha 1 + AMPK alpha 2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
- Isotype: IgG2a
Overview
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Product name
HRP Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2]
See all AMPK alpha 1 + AMPK alpha 2 primary antibodies -
Description
HRP Mouse monoclonal [34.2] to AMPK alpha 1 + AMPK alpha 2 -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Drosophila melanogaster
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Immunogen
Synthetic peptide corresponding to Drosophila melanogaster AMPK alpha 1 + AMPK alpha 2 (N terminal).
Database link: Q13131 -
Positive control
- WB: Rat heart and Human brain tissue lysates; HepG2 and HEK293 whole cell lysates. IHC-P: FFPE Human colon adenocarcinoma.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
34.2 -
Isotype
IgG2a -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab202685)
IHC image of AMPK alpha 1 + AMPK alpha 2 staining in a section of formalin-fixed paraffin-embedded human colon adenocarcinoma*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab202685, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - HRP Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab202685)](https://www.abcam.com/ps/products/202/ab202685/Images/ab202685-248927-wbab202685120m.jpg)
Western blot - HRP Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab202685)All lanes : HRP Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab202685) at 1/5000 dilution
Lane 1 : Heart (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 62 kDa
Observed band size: 62,75,90 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202685 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
