Anti-AMPK alpha 1 + AMPK alpha 2 antibody (ab131512)
Key features and details
- Rabbit polyclonal to AMPK alpha 1 + AMPK alpha 2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-AMPK alpha 1 + AMPK alpha 2 antibody
See all AMPK alpha 1 + AMPK alpha 2 primary antibodies -
Description
Rabbit polyclonal to AMPK alpha 1 + AMPK alpha 2 -
Host species
Rabbit -
Specificity
ab131512 detects endogenous levels of total AMPK alpha 1 + AMPK alpha 2 protein. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide conjugated to KLH from around amino acids 181-185/170-174 (
LRTSC
) of Human AMPK alpha 1 + AMPK alpha 2 (NP_006242.5, NP_006243.2). -
Positive control
- HeLa cells; A549, HepG2 and 3T3 cell extracts; Human breast carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab131512 was purified by affinity chromatography using epitope specific peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody (ab131512)All lanes : Anti-AMPK alpha 1 + AMPK alpha 2 antibody (ab131512) at 1/500 dilution
Lane 1 : A549 cell extract
Lane 2 : HepG2 cell extract
Lane 3 : 3T3 cell extract
Predicted band size: 62 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 + AMPK alpha 2 antibody (ab131512)Immunofluorescent analysis of methanol fixed HeLa cells labelling AMPK alpha 1 + AMPK alpha 2 with ab131512 at 1/100 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 + AMPK alpha 2 antibody (ab131512)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling AMPK alpha 1 + AMPK alpha 2 with ab131512 at 1/50 dilution. The image on the right is treated with the synthesized peptide.
