Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab80039)

Key features and details

  • Mouse monoclonal [34.2] to AMPK alpha 1 + AMPK alpha 2
  • Suitable for: WB
  • Reacts with: Rat, Human, Drosophila melanogaster
  • Isotype: IgG2a

Overview

  • Product name

    Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2]
    See all AMPK alpha 1 + AMPK alpha 2 primary antibodies

  • Description

    Mouse monoclonal [34.2] to AMPK alpha 1 + AMPK alpha 2

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    WB
    Rat
    Human
    Drosophila melanogaster
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Drosophila melanogaster AMPK alpha 1 + AMPK alpha 2 (N terminal).

  • Positive control

    • WB: Rat heart tissue lysate. Human brain tissue lysate. HEK-293 and HepG2 whole cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Images

  • Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab80039)
    Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab80039)
    All lanes : Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab80039) at 1 µg/ml

    Lane 1 : Heart (Rat) Tissue Lysate
    Lane 2 : Human brain tissue lysate - total protein (ab29466)
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 62 kDa
    Observed band size: 62 kDa
    Additional bands at: 75 kDa, 80 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    Abcam recommends using milk as the blocking agent when running western blots of ab80039. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab80039 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.
  • Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab80039)
    Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab80039) This image is courtesy of an Abreview submitted by Brandon d'Eon
    Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab80039) at 1/1000 dilution + Mouse 3T3-L1 adipocytes whole cell lysate at 25 µg

    Secondary
    HRP-conjugated goat anti-mouse IgG (H+L) polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 62 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes

    See Abreview

  • Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab80039)
    Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab80039) Image courtesy of Richelle Sopko, Harvard University, U.S.A.
    All lanes : Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] (ab80039) at 1/1000 dilution

    Lane 1 : Lysate of 0-4 hour Drosophila embryos expressing control shRNA.
    Lane 2 : Lysate of 0-4 hour Drosophila embryos expressing SNF1A shRNA.

    Secondary
    All lanes : Sheep anti-mouse IgG conjugated to HRP at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 62 kDa


    Exposure time: 10 minutes

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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