Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] - BSA and Azide free (ab222491)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19549] to AMPK alpha 1 + AMPK alpha 2 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] - BSA and Azide free
    See all AMPK alpha 1 + AMPK alpha 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR19549] to AMPK alpha 1 + AMPK alpha 2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human AMPK alpha 1 and AMPK alpha 2 full length recombinant proteins; MCF7, HepG2, HeLa, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human skeletal muscle, fetal liver, fetal heart and fetal kidney lysates; Mouse brain and heart lysates; Rat brain, heart and spleen lysates. IP: MCF7 whole cell lysate.

  • General notes

    Ab222491 is the carrier-free version of ab207442. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab222491 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Images

  • Immunoprecipitation - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] - BSA and Azide free (ab222491)
    Immunoprecipitation - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] - BSA and Azide free (ab222491)

    AMPK alpha 1 + AMPK alpha 2 was immunoprecipitated from 0.35 mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab207442 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab207442 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: MCF7 whole cell lysate 10ug (Input).

    Lane 2: ab207442 IP in MCF7 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207442 in MCF7 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207442).

  • Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] - BSA and Azide free (ab222491)
    Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] - BSA and Azide free (ab222491)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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