Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19549] to AMPK alpha 1 + AMPK alpha 2
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human, Recombinant fragment

Product name

Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549]
See all AMPK alpha 1 + AMPK alpha 2 primary antibodies
Description

Rabbit monoclonal [EPR19549] to AMPK alpha 1 + AMPK alpha 2
Host species

Rabbit

Tested Applications & Species

Application	Species
IP	Human
WB	Mouse Rat Human Recombinant fragment

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human AMPK alpha 1 and AMPK alpha 2 full length recombinant proteins; MCF7, HepG2, HeLa, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human skeletal muscle, fetal liver, fetal heart and fetal kidney lysates; Mouse brain and heart lysates; Rat brain, heart and spleen lysates. IP: MCF7 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19549
Isotype

IgG
Research areas

Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)

All lanes : Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442) at 1/2000 dilution

Lane 1 : Human AMPK alpha 1 full length recombinant protein
Lane 2 : Human AMPK alpha 2 full length recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 62 kDa
Observed band size: 62,64 kDa
why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.

Human AMPK alpha 1 and AMPK alpha 2 full length recombinant proteins contains aa1-559 and aa1-552 with a His-Tag®.
Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)

Lanes 1-2 : Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442) at 1/10000 dilution
Lanes 3-4 : Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442) at 1/1000 dilution

Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : Human skeletal muscle lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 62 kDa
Observed band size: 62 kDa

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)

All lanes : Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442) at 1/1000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 62 kDa
Observed band size: 62 kDa

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)

All lanes : Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat heart lysate
Lane 5 : Rat spleen lysate
Lane 6 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 7 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 9 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 62 kDa
Observed band size: 62 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1/2: 30 seconds; Lane 3-5: 3 minutes Lane 6-9: 5 seconds.
Immunoprecipitation - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)

AMPK alpha 1 + AMPK alpha 2 was immunoprecipitated from 0.35 mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab207442 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab207442 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: MCF7 whole cell lysate 10ug (Input).

Lane 2: ab207442 IP in MCF7 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207442 in MCF7 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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