Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19549] to AMPK alpha 1 + AMPK alpha 2
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549]
    See all AMPK alpha 1 + AMPK alpha 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR19549] to AMPK alpha 1 + AMPK alpha 2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human AMPK alpha 1 and AMPK alpha 2 full length recombinant proteins; MCF7, HepG2, HeLa, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human skeletal muscle, fetal liver, fetal heart and fetal kidney lysates; Mouse brain and heart lysates; Rat brain, heart and spleen lysates. IP: MCF7 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)
    Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)
    All lanes : Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442) at 1/2000 dilution

    Lane 1 : Human AMPK alpha 1 full length recombinant protein
    Lane 2 : Human AMPK alpha 2 full length recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 62 kDa
    Observed band size: 62,64 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human AMPK alpha 1 and AMPK alpha 2 full length recombinant proteins contains aa1-559 and aa1-552 with a His-Tag®.

  • Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)
    Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)
    Lanes 1-2 : Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442) at 1/10000 dilution
    Lanes 3-4 : Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442) at 1/1000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : Human skeletal muscle lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 62 kDa
    Observed band size: 62 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)
    Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)
    All lanes : Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 62 kDa
    Observed band size: 62 kDa


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)
    Western blot - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)
    All lanes : Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Rat heart lysate
    Lane 5 : Rat spleen lysate
    Lane 6 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 7 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 9 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 62 kDa
    Observed band size: 62 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/2: 30 seconds; Lane 3-5: 3 minutes Lane 6-9: 5 seconds.

  • Immunoprecipitation - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)
    Immunoprecipitation - Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)

    AMPK alpha 1 + AMPK alpha 2 was immunoprecipitated from 0.35 mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab207442 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab207442 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: MCF7 whole cell lysate 10ug (Input).

    Lane 2: ab207442 IP in MCF7 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207442 in MCF7 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)
    Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)

© 2021 Astana Biomed Group LLP. All rights reserved.