Anti-MAGE3 antibody [EPR19065] - BSA and Azide free (ab251574)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19065] to MAGE3 - BSA and Azide free
- Suitable for: ICC, WB, IP, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-MAGE3 antibody [EPR19065] - BSA and Azide free
See all MAGE3 primary antibodies -
Description
Rabbit monoclonal [EPR19065] to MAGE3 - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody cross reacts with MAGEA2, MAGEA6, MAGEA8 and MAGEA12 and does not cross react with MAGEA1 and MAGEA4.
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Tested applications
Suitable for: ICC, WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251574 is the carrier-free version of ab223162 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab251574 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as MAGEA3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19065 -
Isotype
IgG -
Research areas
Images
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Lane 1 : Anti-MAGE3 antibody [EPR19065] (ab223162) at 1/20000 dilution
Lanes 2 & 4 : Anti-MAGE3 antibody [EPR19065] (ab223162) at 1/10000 dilution
Lane 3 : Anti-MAGE3 antibody [EPR19065] (ab223162) at 1/1000 dilution
Lane 1 : His- and vinc-tagged human MAGEA2 recombinant protein (aa1-314)
Lane 2 : His-tagged human MAGEA6 recombinant protein (aa1-314)
Lane 3 : His-tagged human MAGEA8 recombinant protein (aa1-318)
Lane 4 : His-tagged human MAGEA12 recombinant protein (aa1-314)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 38,41,70 kDa why is the actual band size different from the predicted?This data was developed using ab223162, the same antibody clone in a different buffer formulation.
Exposure time: Lane 1: 3 seconds; Lanes 2, 4: 1 second; Lane 3: 30 seconds.
Blocking and dilution buffer: 5% NFDM/TBST.
Vinc-tag is derived from protein vinculin (P18206) and contains aa1-258 (29 kDa).
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All lanes : Anti-MAGE3 antibody [EPR19065] (ab223162) at 1/1000 dilution
Lane 1 : His-tagged human MAGEA1 recombinant protein (aa1-309)
Lane 2 : His-tagged human MAGEA4 recombinant protein (aa1-317)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 35 kDa
Exposure time: 3 minutesThis data was developed using ab223162, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-MAGE3 antibody [EPR19065] (ab223162) at 1/5000 dilution
Lane 1 : A-375 (human malignant melanoma cell line) whole cell lysate
Lane 2 : RPMI-8226 (human plasmacytoma, myeloma B lymphocyte cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 35 kDaThis data was developed using ab223162, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-2: 5 seconds; Lane 3: 2 seconds.
The observed molecular weight is consistent with what has been described in the literature (PMID: 7757970).
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This data was developed using ab223162, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human testis tissue labeling MAGE3 with ab223162 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on germ cells of human testis (PMID: 23390371). Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
This data was developed using ab223162, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling MAGE3 with ab223162 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining on human colon cancer (PMID: 23390371). Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
This data was developed using ab223162, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling MAGE3 with ab223162 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human glioma (PMID: 23946777). Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
This data was developed using ab223162, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human melanoma tissue labeling MAGE3 with ab223162 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining on human melanoma (PMID: 26100270). Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
This data was developed using ab223162, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling MAGE3 with ab223162 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining on human gastric cancer (PMID: 16158951). Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
This data was developed using ab223162, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling MAGE3 with ab223162 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Negative control: No staining on human stomach (PMID: 16158951). Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
This data was developed using ab223162, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling MAGE3 with ab223162 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on HeLa cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594 (ab195889) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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This data was developed using ab223162, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol-fixed A-375 (human malignant melanoma cell line) cells labeling MAGE3 with ab223162 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on A-375 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594 (ab195889) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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This data was developed using ab223162, the same antibody clone in a different buffer formulation.
MAGE3 was immunoprecipitated from 0.35 mg of A-375 (human malignant melanoma cell line) whole cell lysate with ab223162 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223162 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: A-375 whole cell lysate 10 µg (Input).
Lane 2: ab223162 IP in A-375 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223162 in A-375 whole cell lysate
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
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