Anti-Ficolin 2 antibody [EPR23146-225] - BSA and Azide free (ab270404)
![Anti-Ficolin 2 antibody [EPR23146-225] - BSA and Azide free (ab270404)](https://www.abcam.com/ps/products/270/ab270404/Images/ab270404-381982-AntiFicolin-2-antibody-EPR23146225-immunoprecipitation-human.jpg "Anti-Ficolin 2 antibody [EPR23146-225] - BSA and Azide free (ab270404)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23146-225] to Ficolin 2 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Human
Overview
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Product name
Anti-Ficolin 2 antibody [EPR23146-225] - BSA and Azide free
See all Ficolin 2 primary antibodies -
Description
Rabbit monoclonal [EPR23146-225] to Ficolin 2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human plasma; human liver lysate; Human Ficolin-2 recombinant protein (aa26- 313). IP: Human plasma.
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General notes
ab270404 is the carrier-free version of ab267473. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23146-225 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Ficolin 2 antibody [EPR23146-225] - BSA and Azide free (ab270404)
Ficolin 2 was immunoprecipitated from 0.35 mg Human plasma with ab267473 at 1/30 dilution . Western blot was performed on the immunoprecipitate using ab267473 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP(ab131366) was used at 1/5000 dilution.
Lane 1: Human plasma lysate 10 μg.
Lane 2: ab267473 IP in Human plasma.
Lane 3: Rabbit monoclonal IgG isotype control (ab172730) instead of ab267473 in Human plasma.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 7.75 seconds
Ficolin 2 has a high molecular weight (250 kDa) due to extensive disulfide bridge formation (PMID: 16595153).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (abXXX).
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![Anti-Ficolin 2 antibody [EPR23146-225] - BSA and Azide free (ab270404)](https://www.abcam.com/ps/products/270/ab270404/Images/ab270404-2-benefits-of-recombinant-antibodies.png)
Anti-Ficolin 2 antibody [EPR23146-225] - BSA and Azide free (ab270404)
