Human Transferrin ELISA Kit (ab108911)
Key features and details
- Sensitivity: 0.085 µg/ml
- Range: 0.098 µg/ml - 25 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Human Transferrin ELISA Kit
See all Transferrin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.9% Inter-assay Sample n Mean SD CV% Overall 10.1% -
Sample type
Serum, Plasma -
Assay type
Competitive -
Sensitivity
= 0.085 µg/ml -
Range
0.098 µg/ml - 25 µg/ml -
Recovery
99 %
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Assay time
2h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Transferrin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Transferrin in plasma and serum samples.
A Transferrin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Transferrin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Transferrin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml 6X Biotinylated Human Transferrin (Lyophilized) 1 vial Chromogen Substrate 1 x 7ml Human Transferrin Standard (Lyophilized) 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml Transferrin Microplate (12 x 8 well strips) 1 unit -
Research areas
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Function
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. -
Sequence similarities
Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Apotransferrin
- Beta 1 metal binding globulin
- Beta-1 metal-binding globulin
see all -
Database links
- Entrez Gene: 7018 Human
- Omim: 190000 Human
- SwissProt: P02787 Human
- Unigene: 518267 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab108911
