Anti-MIF antibody (ab55445)
Key features and details
- Mouse monoclonal to MIF
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-MIF antibody
See all MIF primary antibodies -
Description
Mouse monoclonal to MIF -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant full length protein, corresponding to amino acids 1-116 of Human MIF
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Positive control
- IHC-P: Human lung cell carcinoma tissue. Flow cyt: Jurkat cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 24/1/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4 -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-MIF antibody (ab55445)
Western blot against tagged recombinant protein immunogen using ab55445 MIF antibody at 1μg/ml. Predicted band size of immunogen is 38 kDa
This image was generated using the ascites version of the product.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody (ab55445)MIF antibody (ab55445) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human lung, adenosqumous cell carcinoma tissue. -
Flow Cytometry - Anti-MIF antibody (ab55445)Overlay histogram showing Jurkat cells stained with ab55445 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55445, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody (ab55445)Paraffin embedded human lung squamous cell carcinoma tissue stained for MIF using ab55445 at 6 µg/ml in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody (ab55445) Image from Li J et al., J Biol Chem. 2012 Oct 12;287(42):35484-95. doi: 10.1074/jbc.M112.367532. Epub 2012 Aug 14.Fig 7.; October 12, 2012 The Journal of Biological Chemistry, 287, 35484-35495.Immunohistochemical analysis of Human nasopharyngeal carcinoma, staining MIF with ab55445 at 1/100 dilution.
This image was generated using the ascites version of the product.
