Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LBP antibody [EPR22559-261] (ab254559)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22559-261] to LBP
  • Suitable for: IP, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-LBP antibody [EPR22559-261]
    See all LBP primary antibodies

  • Description

    Rabbit monoclonal [EPR22559-261] to LBP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse serum and plasma lysate; SP2/0-Ag14 whole cell lysate; Mouse ovary tissue lysate; His-tagged full-length recombinant mouse LBP protein. IP: Mouse ovary lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunoprecipitation - Anti-LBP antibody [EPR22559-261] (ab254559)
    Immunoprecipitation - Anti-LBP antibody [EPR22559-261] (ab254559)

    LBP was immunoprecipitated from 0.35 mg mouse ovary lysate with ab254559 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab254559 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

    Lane 1: Mouse ovary lysate 10μg (Input).
    Lane 2: ab254559 IP in mouse ovary lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254559 in mouse ovary lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 20 seconds.

    This blot was developed using a higher sensitivity ECL substrate.

  • Western blot - Anti-LBP antibody [EPR22559-261] (ab254559)
    Western blot - Anti-LBP antibody [EPR22559-261] (ab254559)
    All lanes : Anti-LBP antibody [EPR22559-261] (ab254559) at 1/1000 dilution

    Lane 1 : Mouse serum
    Lane 2 : Mouse plasma

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?



    Blocking/diluting buffer and concentration: 5% NFDM/TBST 

    Exposure time: 59 seconds

    The expression profile / molecular weight observed is consistent with what has been described in the literature (PMID:7694297).

  • Western blot - Anti-LBP antibody [EPR22559-261] (ab254559)
    Western blot - Anti-LBP antibody [EPR22559-261] (ab254559)
    All lanes : Anti-LBP antibody [EPR22559-261] (ab254559) at 1/1000 dilution

    Lane 1 : SP2/0-Ag14 (mouse myeloma cell line), whole cell lysate
    Lane 2 : Mouse ovary tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?



    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 59 seconds

    The expression profile / molecular weight observed is consistent with what has been described in the literature (PMID:7694297)

    Negative control: SP2/0-Ag14 (PMID: 2456339).

  • Western blot - Anti-LBP antibody [EPR22559-261] (ab254559)
    Western blot - Anti-LBP antibody [EPR22559-261] (ab254559)
    Anti-LBP antibody [EPR22559-261] (ab254559) at 1/1000 dilution + His-tagged full-length recombinant mouse LBP protein 10 ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?



    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Anti-LBP antibody [EPR22559-261] (ab254559)
    Anti-LBP antibody [EPR22559-261] (ab254559)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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