Antibodies, Proteins, Kits and Reagents for Life Science

334 369 ₸ Product size

100 µl 334 369 ₸

Order now and get it on Wednesday March 03, 2021

HRP Anti-MIF antibody [EPR12463] (ab196645)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
HRP Rabbit monoclonal [EPR12463] to MIF
Suitable for: IHC-P, WB
Knockout validated
Reacts with: Human
Conjugation: HRP

Product name

HRP Anti-MIF antibody [EPR12463]
See all MIF primary antibodies
Description

HRP Rabbit monoclonal [EPR12463] to MIF
Host species

Rabbit
Conjugation

HRP
Tested applications

Suitable for: IHC-P, WBmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Y70 whole cell lysate and Human Fetal Brain tissue lysate. IHC-P: Normal human lung tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12463
Isotype

IgG
Research areas

Western blot - HRP Anti-MIF antibody [EPR12463] (ab196645)

All lanes : HRP Anti-MIF antibody [EPR12463] (ab196645) at 2 µg/ml

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : MIF knockout HAP1 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 12 kDa
Observed band size: 12 kDa

ab196645 was shown to recognize MIF (Macrophage migration inhibitory factor) in wild-type HAP1 cells as signal was lost at the expected MW in MIF knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and MIF knockout samples were subjected to SDS-PAGE. Ab196645 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 2 μg/ml and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
Western blot - HRP Anti-MIF antibody [EPR12463] (ab196645)

All lanes : HRP Anti-MIF antibody [EPR12463] (ab196645) at 1/2000 dilution

Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 2 : Brain (Human) Tissue Lysate - fetal normal tissue (ab29467)

Lysates/proteins at 10 µg per lane.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 12 kDa
Observed band size: 12 kDa

Exposure time: 4 minutes

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab196645 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-MIF antibody [EPR12463] (ab196645)

IHC image of MIF staining in a section of formalin-fixed paraffin-embedded normal human lung, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196645 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
HRP Anti-MIF antibody [EPR12463] (ab196645)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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