Chitotriosidase Assay Kit (ab241009)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
Overview
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Product name
Chitotriosidase Assay Kit
See all CHIT1 kits -
Detection method
Fluorescent -
Product overview
Chitotriosidase Assay Kit (ab241009) utilizes a fluorogenic substrate that can be hydrolyzed by chitinase and a set of proprietary assay buffers that can distinguish specific CHIT1 activity from other hydrolases including Acidic Mammalian Chitinase. The kit provides a simple, specific, sensitive assay that can detect as low as 0.2 mU/mL of CHIT1 in a variety of biological samples.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 4-Methylumbelliferone Standard (5 mM) 1 x 35µl CHIT1 Assay Buffer 1 x 25ml CHIT1 Inhibition Buffer 1 x 18ml CHIT1 Substrate (in DMSO) 1 x 25µl Chitotriosidase (lyophilized) 1 vial -
Research areas
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Function
Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity. -
Tissue specificity
Detected in spleen. Secreted by cultured macrophages. -
Sequence similarities
Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.
Contains 1 chitin-binding type-2 domain. -
Cellular localization
Secreted. Lysosome. A small proportion is lysosomal. - Information by UniProt
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Alternative names
- CHI3
- CHIT
- CHIT1
see all
Images
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4-Methylumbelliferon Standard Curve.
4-Methylumbelliferon Standard Curve.
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Measurement of purified Human Chitiotriosidase activity with or without Inhibition BufferMeasurement of purified Human Chitiotriosidase activity with or without Inhibition Buffer
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Measurement of CHIT1 activityMeasurement of CHIT1 activity in Human Plasma (4 µL), Human Serum (4 µL), U937 Cell Extract (10 µg) and J774 Cell Extract (10 µg). All assays were performed following kit protocol.