Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)
![Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)](https://www.abcam.com/ps/products/21/ab21356/Images/ab21356-30367-anti-lipoprotein-lipase-antibody-lpla4-western-blot.jpg "Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)")
Key features and details
- Mouse monoclonal [LPL.A4] to Lipoprotein lipase
- Suitable for: Flow Cyt, WB
- Reacts with: Cow, Human
- Isotype: IgG1
Overview
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Product name
Anti-Lipoprotein lipase antibody [LPL.A4]
See all Lipoprotein lipase primary antibodies -
Description
Mouse monoclonal [LPL.A4] to Lipoprotein lipase -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Full length protein (Human).
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Positive control
- Purified human and bovine recombinant Lipoprotein lipase.
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General notes
This product was changed from ascites to tissue culture supernatant on 25th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Protein A affinity chromatography -
Clonality
Monoclonal -
Clone number
LPL.A4 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)All lanes : Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356) at 1/1000 dilution
Lane 1 : Human Lipoprotein lipase.
Lane 2 : Bovine Lipoprotein lipase
Lysates/proteins at 10 µg per lane.
Predicted band size: 53 kDa
Observed band size: 55,60 kDa why is the actual band size different from the predicted?
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![Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)](https://www.abcam.com/ps/products/21/ab21356/Images/ab21356-30366-anti-lipoprotein-lipase-antibody-lpla4-flow-cytometry.jpg)
Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)Overlay histogram showing HeLa cells stained with ab21356 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab21356, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
