Heparin Sepharose® (ab193268)
Overview
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Product name
Heparin Sepharose® -
Product overview
High binding capacity (>0.4mg/mL). Minimal leaching of ligand. For column or batch purification of heparin-binding proteins & cation exchange (ab193268).
Contents:
Supplied as a 50% slurry in 20 % Ethanol; >2.5 mg heparin per mL Sepharose® beads.
Features:
Heparin beads have been widely used in affinity purification of various heparin-binding proteins or ligands, such as antithrombin III, lipoprotein, as well as DNA binding proteins (transcription factors, virus coat proteins etc). Abcam’s Heparin Sepharose® is designed for purification of heparin-binding proteins and ligands. It can also be used as a high capacity cation exchange medium. Specific proteins can be separated by using different concentrations of salt or a salt gradient. This Heparin Sepharose® formulation exhibits excellent binding capacity, high flow rate, no significant loss of the heparin ligand and a pH stability range of 2-10.
These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x g when centrifuging.
Store beads at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:
Purification of heparin-binding proteins, enzymes or other ligands.
Sepharose is a registered trademark of GE Healthcare
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Notes
Heparin Sepharose® is prepared by covalently coupling heparin to epoxy-activated 6% cross-linked Sepharose® beads. The coupling was optimized to give a high binding capacity and could be greater than 0.4 mg of heparin-binding protein (such as thrombin) per ml of wet gel.
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Tested applications
Suitable for: Purificationmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 ml 10 ml 50 ml Heparin Sepharose® 1 x 1ml 1 x 10ml 1 x 50ml -
Research areas
