Antibodies, Proteins, Kits and Reagents for Life Science

Anti-TLR3 antibody [EPR24454-103] (ab281560)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR24454-103] to TLR3
Suitable for: Flow Cyt (Intra)
Reacts with: Human

Product name

Anti-TLR3 antibody [EPR24454-103]
See all TLR3 primary antibodies
Description

Rabbit monoclonal [EPR24454-103] to TLR3
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra)more details
Unsuitable for: ICC,IHC-P,IP or WB
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Flow cyt: Human PBMC treated with 100ng/ml GM-CSF and 50ng/ml IL-4 for 6 days.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR24454-103
Isotype

IgG

Flow Cytometry - Anti-TLR3 antibody [EPR24454-103] (ab281560)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Human peripheral blood mononuclear cell (PBMC) treated with 100ng/ml GM-CSF and 50ng/ml IL-4 for 6 days cells labelling TLR3 with ab281560 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with anti-CD11c conjugated to Alexa Fluor® 647 and anti-HLA-DR conjugated to PE, then stained with rabbit IgG or ab281560. Gated on HLA-DR+ population.
Flow Cytometry - Anti-TLR3 antibody [EPR24454-103] (ab281560)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Human peripheral blood mononuclear cell (PBMC) treated with 100ng/ml GM-CSF and 50ng/ml IL-4 for 6 days cells labelling TLR3 with ab281560 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Anti-TLR3 antibody [EPR24454-103] (ab281560)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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