Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12462] to MIF
Suitable for: WB, IP
Knockout validated
Reacts with: Human

Product name

Anti-MIF antibody [EPR12462]
See all MIF primary antibodies
Description

Rabbit monoclonal [EPR12462] to MIF
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human MIF aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P14174
Positive control
- Y79, Jurkat, A375 and THP1 cell lysates.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12462
Isotype

IgG
Research areas

Western blot - Anti-MIF antibody [EPR12462] (ab176565)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: MIF knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab176565 observed at 12 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab176565 was shown to specifically recognize MIF in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when knockout cells were examined. Wild-type and MIF knockout samples were subjected to SDS-PAGE. Ab176565 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-MIF antibody [EPR12462] (ab176565)

All lanes : Anti-MIF antibody [EPR12462] (ab176565) at 1/1000 dilution

Lane 1 : Y79 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : A375 cell lysate
Lane 4 : THP1 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 12 kDa
Immunoprecipitation - Anti-MIF antibody [EPR12462] (ab176565)

Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate labeling MIF with ab176565 at 1/10 dilution.
Anti-MIF antibody [EPR12462] (ab176565)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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