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Rat NLRP3 Antibody Pair - BSA and Azide free (ab253473)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Rat

Overview

  • Product name

    Rat NLRP3 Antibody Pair - BSA and Azide free
    See all NLRP3 kits

  • Assay type

    ELISA set

  • Range

    15.625 pg/ml - 1000 pg/ml

  • Species reactivity

    Reacts with: Rat

  • Product overview

    Both capture and detector antibodies are recombinant rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    The Antibody Pair can be used to quantify Rat NLRP3. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab277086), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.

  • Carrier free

    Yes
  • Components 10 x 96 tests
    Rat NLRP3 Capture Antibody (unconjugated) 1 x 100µg
    Rat NLRP3 Detector Antibody (unconjugated) 1 x 100µg

  • Research areas

  • Function

    May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.

  • Tissue specificity

    Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder.

  • Involvement in disease

    Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold.
    Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.
    Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation.

  • Sequence similarities

    Belongs to the NLRP family.
    Contains 1 DAPIN domain.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Alternative names

    • AGTAVPRL
    • AII/AVP
    • Angiotensin/vasopressin receptor AII/AVP like
    • Angiotensin/vasopressin receptor AII/AVP-like
    • C1orf7
    • Caterpiller protein 1.1
    • CIAS 1
    • CIAS1
    • CLR1.1
    • Cold autoinflammatory syndrome 1
    • Cold autoinflammatory syndrome 1 protein
    • Cryopyrin
    • Familial cold autoinflammatory syndrome
    • FCAS
    • FCU
    • LRR and PYD domains-containing protein 3
    • Muckle-Wells syndrome
    • MWS
    • NACHT
    • NACHT LRR and PYD containing protein 3
    • NALP 3
    • NALP3
    • NALP3_HUMAN
    • NLR family pyrin domain containing 3
    • NLRP3
    • PYPAF 1
    • PYPAF1
    • PYRIN containing APAF1 like protein 1
    • PYRIN-containing APAF1-like protein 1
    see all

  • Database links

    Images

    • Sandwich ELISA - Rat NLRP3 Antibody Pair - BSA and Azide free (ab253473)
      Sandwich ELISA - Rat NLRP3 Antibody Pair - BSA and Azide free (ab253473)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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