Anti-P97/DAP5 antibody (ab97302)
Key features and details
- Rabbit polyclonal to P97/DAP5
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-P97/DAP5 antibody
See all P97/DAP5 primary antibodies -
Description
Rabbit polyclonal to P97/DAP5 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow -
Immunogen
Recombinant fragment corresponding to Human P97/DAP5 aa 1-204.
Database link: P78344 -
Positive control
- WB: A431 whole cell lysate. Mouse brain whole cell lysate. IHC-P: Cal27 xenograft, human skeletal muscle. Mouse hind brain tissue. IF/ICC: HeLa cell line.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-P97/DAP5 antibody (ab97302) at 1/500 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 102 kDa5% SDS PAGE
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Immunohistochemical analysis of paraffin-embedded Cal27 xenograft using ab97302 at 1/100 dilution
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HeLa cells stained for EIF4G2 (green) using ab97302 at 1/500 dilution in ICC/IF.
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Paraffin embedded mouse hind brain tissue stained for DAP5 using ab97302 at 1/500 dilution in immunohistochemical analysis.
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Anti-P97/DAP5 antibody (ab97302) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 102 kDa
7.5% SDS PAGE -
ICC/IF image of ab97302 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97302, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Paraffin embedded human skeletal muscle tissue stained for DAP5 using ab97302 at 10 μg/ml in immunohistochemical analysis.