Mouse TECK ELISA Kit (CCL25) (ab269560)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 7.4 pg/ml
- Range: 12.5 pg/ml - 800 pg/ml
- Sample type: Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse TECK ELISA Kit (CCL25)
See all CCL25 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 3 2% Inter-assay Sample n Mean SD CV% Extract 8 2.2% -
Sample type
Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
7.4 pg/ml -
Range
12.5 pg/ml - 800 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 99 89% - 114% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse TECK ELISA Kit (CCL25) (ab269560) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TECK (CCL25) protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse TECK (CCL25) with 7.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Mouse TECK (CCL25) is a CC chemokine produced by stromal cells in the thymus and epithelial cells of the small intestine. TECK is involved in human T-cell development inducing chemoattraction through CCR9 on immature pre-T cells and thymocytes. TECK induces the homing of several lymphocyte populations to the small intestine, including integrin a4b7+ gamma/delta T cells, Integrin aEb7+ CD8+ T cells, and IgA-producing plasma cells. In human cancer, TECK promotes the metastasis of melanoma cells, migration and invasion of some breast carcinomas, and mesenchymal stromal cells in multiple myelomas. TECK attracts CCR9+ monocytes and macrophages in rheumatoid arthritis and CCR9+ macrophages in atherosclerosis. Mouse TECK shares 50% ,81% and 61 % sequence homology with mouse, rat and cow, respectively
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse TECK (CCL25) Capture Antibody 1 x 600µl 10X Mouse TECK (CCL25) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Mouse TECK (CCL25) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. -
Tissue specificity
Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- A130072A22Rik
- C-C motif chemokine 25
- CCL 25
see all -
Database links
- Entrez Gene: 20300 Mouse
- SwissProt: O35903 Mouse
- Unigene: 7275 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse TECK standard curve in 1X Cell Extraction Buffer PTR.The TECK standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native TECK in mouse thymus extract samples based on a 75 µg/mL extract load.The concentrations of TECK were measured in duplicate and interpolated from the TECK standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TECK concentration was determined to be 658 pg/mL in thymus extract.
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Sandwich ELISA - Mouse TECK ELISA Kit (CCL25) (ab269560)To learn more about the advantages of recombinant antibodies see here.
