Anti-VIP2 antibody (ab183527)
Key features and details
- Rabbit polyclonal to VIP2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-VIP2 antibody
See all VIP2 primary antibodies -
Description
Rabbit polyclonal to VIP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Guinea pig, Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan
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Immunogen
Synthetic peptide within Human VIP2 aa 925-975. The exact sequence is proprietary.
Database link: O43314 -
Positive control
- K562, HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab183527 was affinity purified using an epitope specific to VIP2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-VIP2 antibody (ab183527)
Detection of VIP2 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab183527 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab183527 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
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Western blot - Anti-VIP2 antibody (ab183527)All lanes : Anti-VIP2 antibody (ab183527) at 0.4 µg/ml
Lane 1 : K562 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : 293T whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : Mouse TCMK-1 whole cell lysate
Lane 6 : Mouse NIH-3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 140 kDa
Exposure time: 10 seconds
