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Anti-PI3K p85 (phospho Y458) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (ab278545)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [PI3KY458-1A11] to PI3K p85 (phospho Y458) + PI3 Kinase p55 (phospho Y199)
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PI3K p85 (phospho Y458) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11]

  • Description

    Rabbit monoclonal [PI3KY458-1A11] to PI3K p85 (phospho Y458) + PI3 Kinase p55 (phospho Y199)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    Human
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PI3K p85 (phospho Y458). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q92569

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: UV-treated NIH/3T3 cell extracts. Flow Cyt: Ramos and NIH/3T3 cells (both treated with pervanadate).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-PI3K p85 (phospho Y458) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (ab278545)
    Western blot - Anti-PI3K p85 (phospho Y458) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (ab278545)
    All lanes : Anti-PI3K p85 (phospho Y458) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (ab278545) at 0.5 µg/ml

    Lane 1 : UV-treated NIH/3T3 (mouse embryo fibroblast cell line) cell extract
    Lane 2 : Untreated NIH/3T3 cell extract

    Predicted band size: 84 kDa

  • Flow Cytometry - Anti-PI3K p85 (phospho Y458) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (ab278545)
    Flow Cytometry - Anti-PI3K p85 (phospho Y458) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (ab278545)

    Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using ab278545 at 0.1 µg/ml.

  • Flow Cytometry - Anti-PI3K p85 (phospho Y458) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (ab278545)
    Flow Cytometry - Anti-PI3K p85 (phospho Y458) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (ab278545)

    Flow cytometric analysis of NIH/3T3 (mouse embryo fibroblast cell line) cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control (orange) or treated with imatinib (red) or with pervanadate (green) using ab278545 at 0.1 µg/ml.

  • Flow Cytometry - Anti-PI3K p85 (phospho Y458) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (ab278545)
    Flow Cytometry - Anti-PI3K p85 (phospho Y458) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (ab278545)

    Peptide blocking flow cytometric analysis of NIH/3T3 (mouse embryo fibroblast cell line) cells secondary antibody only negative control (light blue) or treated with imatinib (red) or with pervanadate (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using ab278545 at 0.1 µg/ml.

  • Anti-PI3K p85 (phospho Y458) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (ab278545)
    Anti-PI3K p85 (phospho Y458) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (ab278545)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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