Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17897] to Histone H3 (mono methyl K122) - BSA and Azide free
  • Suitable for: PepArr, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free
    See all Histone H3 primary antibodies

  • Description

    Rabbit monoclonal [EPR17897] to Histone H3 (mono methyl K122) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: PepArr, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249956 is the carrier-free version of ab177300. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249956 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17897

  • Isotype

    IgG

Images

  • Peptide Array - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956)
    Peptide Array - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956)

    This data was developed using ab177300, the same antibody clone in a different buffer formulation.

    ab177300 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
  • Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956)
    Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956)
    All lanes : Anti-ASPHD1 antibody - N-terminal (ab177330) at 1/5000 dilution

    Lane 1 : HeLa cytosolic fraction
    Lane 2 : HeLa nuclear fraction

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 30 seconds


    This data was developed using ab177300, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% BSA/TBST.

  • Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956)
    Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956)
    Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300) at 1/5000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) histone extraction lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 15 seconds


    This data was developed using ab177300, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% BSA/TBST.

  • Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956)
    Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956)
    Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300) at 1/5000 dilution + NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 30 seconds


    This data was developed using ab177300, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% BSA/TBST.

  • Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956)
    Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956)
    All lanes : Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate: untreated, asynchronous cells
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate: G1/S arrested cells (thymidine treatment)
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate: G2/M arrested cells (sequential thymidine and nocodazole treatments)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 30 seconds


    This data was developed using ab177300, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% BSA/TBST.

  • Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956)
    Anti-Histone H3 (mono methyl K122) antibody [EPR17897] - BSA and Azide free (ab249956)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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