Human Cardiac Troponin I ELISA Kit (ab200016)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4.4 pg/ml
- Range: 31.3 pg/ml - 4000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Cardiac Troponin I Capture Antibody 1 x 600µl 10X Human Cardiac Troponin I Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Cardiac Troponin I Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Cellular localization
Cytosol; Sarcomere. -
Alternative names
- Cardiomyopathy dilated 2A (autosomal recessive)
- CMD1FF
- CMD2A
see all -
Database links
- Entrez Gene: 7137 Human
- Omim: 191044 Human
- SwissProt: P19429 Human
Images
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Other - Human Cardiac Troponin I ELISA Kit (ab200016)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human Cardiac Troponin 1 Standard Curve Comparison between current and original ELISA kit.Standard Curve comparison between current human Cardiac Troponin 1 SimpleStep ELISA kit and original ELISA kit. The current SimpleStep ELISA kit shows increased sensitivity.
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Example of human Cardiac Troponin 1 standard curve in Sample Diluent NS.The Cardiac Troponin 1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Standard curveExample of human Cardiac Troponin 1 standard curve in Sample Diluent NS. The Cardiac Troponin 1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human Cardiac Troponin 1 standard curve in Sample Diluent 50BP.The Cardiac Troponin 1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Standard curveExample of human Cardiac Troponin 1 standard curve in Sample Diluent 50BP. The Cardiac Troponin 1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human Cardiac Troponin 1 standard curve in 1X Cell Extraction Buffer PTR.The Cardiac Troponin 1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Standard curveExample of human Cardiac Troponin 1 standard curve in 1X Cell Extraction Buffer PTR. The Cardiac Troponin 1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Cardiac Troponin 1 in human heart tissue extract based on a 10 µg/mL extract load.The concentrations of Cardiac Troponin 1 were measured in duplicate and interpolated from the Cardiac Troponin 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cardiac Troponin 1 concentration was determined to be 1,793 pg/mL in human heart tissue extract.
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Comparison of Cardiac Troponin 1 in various human tissue extracts.Human heart was analyzed based on a 10 µg/mL extract load while human skeletal muscle and human brain extracts were analyzed based on a 200 µg/mL extract load. The concentrations of Cardiac Troponin 1 were measured in duplicate and interpolated from the Cardiac Troponin 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cardiac Troponin 1 concentration was determined to be 182.4 ng/mg in human heart tissue extract and undetectable in human skeletal muscle and brain tissue extracts.
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Linearity of dilution.Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Recombinant Cardiac Troponin 1 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent 50BP for serum and plasma samples and Sample Diluent NS for cell culture media samples.
Neat pooled serum and plasma (EDTA, Heparin, Citrate) samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.
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Linearity of dilution.Neat serum from ten individual healthy human female donors was measured in duplicate. All values were below the detectable range of the assay.
Native Cardiac Troponin 1 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in 1X Cell Extraction Buffer PTR. -
Sensitivity -
SensitivityThe MDD was determined by calculating the mean of zero standard replicates and adding 2 standard deviations then extrapolating the corresponding concentration.
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Sandwich ELISA - Human Cardiac Troponin I ELISA Kit (ab200016)To learn more about the advantages of recombinant antibodies see here.
