Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (ab56301)
![Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (ab56301)](https://www.abcam.com/ps/products/56/ab56301/Images/ab56301-69552-anti-angiopoietin-2-antibody-mm0020-1f29-immunohistochemistry.jpg "Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (ab56301)")
Key features and details
- Mouse monoclonal [MM0020-1F29] to Angiopoietin 2/ANG2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG2
Overview
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Product name
Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29]
See all Angiopoietin 2/ANG2 primary antibodies -
Description
Mouse monoclonal [MM0020-1F29] to Angiopoietin 2/ANG2 -
Host species
Mouse -
Specificity
This antibody recognizes Angiopoietin 2/ANG2 in western blot without cross reactivity with Angiopoietin 1.
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Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Angiopoietin 2/ANG2.
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Positive control
- Human endothelial cell lysate.
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General notes
This product was previously labelled as Angiopoietin 2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
The IgG fraction of the tissue culture supernatant was purified and then lyophilized from 0.2 µm filtered PBS solution. -
Clonality
Monoclonal -
Clone number
MM0020-1F29 -
Isotype
IgG2 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (ab56301)
ab56301 at 1/200 dilution, staining Angiopoietin 2/ANG2 in human placental tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
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![Western blot - Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (ab56301)](https://www.abcam.com/ps/products/56/ab56301/Images/ab56301-69551-anti-angiopoietin-2-antibody-mm0020-1f29-western-blot.jpg)
Western blot - Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (ab56301)Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (ab56301) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 47 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesThe band observed at 52 kDa could potentially be a cleaved form of Angiopoietin 2/ANG2 due to the presence of an 18 amino acid signal peptide.
